Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

407141 D-myo-Inositol 1,3,5-Trisphosphate Hexakisacetoxymethyl Ester, 2,4,6-Tri-O-butyryl-, - Calbiochem

D-myo-Inositol 1,3,5-Trisphosphate Hexakisacetoxymethyl Ester, 2,4,6-Tri-O-butyryl- is a cell-permeable derivative of Ins(1,3,5)P3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Bt₃(1,3,5)IP₃/AM

407141
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₃₆H₅₇O₃₀P₃
Description
Overview

This product has been discontinued.



A cell-permeable derivative of D-myo-inositol 1,3,5-trisphosphate [Ins(1,3,5)P3].
Note: Each set contains 1 µmol Bt3(1,3,5)IP3/AM and 10 mg PLURONIC® F-127 detergent (Cat. No. 540025) to aid in the solubilization of this product.
Catalogue Number407141
Brand Family Calbiochem®
SynonymsBt₃(1,3,5)IP₃/AM
References
ReferencesRudolf, M.T., et al. 2003. Bioorg. Med. Chem. 11, 3315.
Product Information
ATP CompetitiveN
FormClear syrup
Formulation1 µmol Bt₃(1,3,5)IP₃/AM and 10 mg PLURONIC® F-127 detergent.
Hill FormulaC₃₆H₅₇O₃₀P₃
Chemical formulaC₃₆H₅₇O₃₀P₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationD-myo-Inositol 1,3,5-Trisphosphate Hexakisacetoxymethyl Ester, 2,4,6-Tri-O-butyryl- is a cell-permeable derivative of Ins(1,3,5)P3.
Biological Information
Primary TargetA cell-permeable derivative of D-myo-inositol 1,3,5-trisphosphate [Ins(1,3,5)P3]
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution of IPx, aliquot and freeze (-20°C). Stock solutions of IPx are stable for up to 3 months. Following reconstitution of PLURONIC® F-127 detergent, refrigerate (4°C). DO NOT FREEZE!
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407141 0

Documentation

D-myo-Inositol 1,3,5-Trisphosphate Hexakisacetoxymethyl Ester, 2,4,6-Tri-O-butyryl-, - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

D-myo-Inositol 1,3,5-Trisphosphate Hexakisacetoxymethyl Ester, 2,4,6-Tri-O-butyryl-, - Calbiochem Certificates of Analysis

TitleLot Number
407141

References

Reference overview
Rudolf, M.T., et al. 2003. Bioorg. Med. Chem. 11, 3315.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-February-2008 JSW
SynonymsBt₃(1,3,5)IP₃/AM
DescriptionNovel analogs capable of exhibiting Ins(1,4,5)P3-like agonist or antagonist effects on calcium release are more resistant to hydrolysis and metabolic degradation than IP3 or IP4. Consequently, they can produce a prolonged activation of cellular processes dependent on the Ins(1,4,5)P3 receptor interaction. These tools are important in the elucidation of the molecular interactions of Ins(1,4,5)P3 with its intracellular receptor and with Ins(1,4,5)P3-kinase and 5-phosphatase. Acetoxymethyl esters of inositol phosphate are highly lipophilic. Hence, they easily diffuse through the cell membrane. Note: Each set contains 1 µmol of Bt3(1,3,5)IP3/AM and 10 mg of PLURONIC® F-127 detergent (Cat. No. 540025) to aid in the solubilization of this product.
FormClear syrup
Formulation1 µmol Bt₃(1,3,5)IP₃/AM and 10 mg PLURONIC® F-127 detergent.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions
Protocol for Treating Cells with IPx 1. Add 10 µl DMSO to 1 µmol Bt3(1,3,5)IP3/AM to make a 100 mM stock solution. 2. Prepare a 10% PLURONIC® F-127 solution in DMSO. DO NOT FREEZE! 3. Obtain 1 ml uniform cell suspension and place 500 µl of the suspension in a test tube. 4. Add 1 µl of the DMSO/Bt3(1,3,5)IP3/AM solution (step 1) and 2 µl of the 10% PLURONIC®/DMSO stock solution (step 2) to the cell suspension in step 3 and mix. 5. Add the cells/Bt3(1,3,5)IP3/AM /PLURONIC® mixture from step 4 to a tube containing the remaining 500 µl cell suspension. Mix carefully. The total test-tube volume now equals 1 ml.
Chemical formulaC₃₆H₅₇O₃₀P₃
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (IPx, 100 mM; PLURONIC® F-127 detergent, 100 mg/ml)
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution of IPx, aliquot and freeze (-20°C). Stock solutions of IPx are stable for up to 3 months. Following reconstitution of PLURONIC® F-127 detergent, refrigerate (4°C). DO NOT FREEZE!
Toxicity Standard Handling
ReferencesRudolf, M.T., et al. 2003. Bioorg. Med. Chem. 11, 3315.