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14-487M Cdk3/Cyclin E Protein, active, 250 µg

Active complex of recombinant full length human Cdk3 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag, for use in Kinase Assays.

Cdk3/Cyclin E Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-487M
250 µg  Also available in 10 μg size (cat#14-487) and in bulk (cat# 14-487-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-487M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk3/Cyclin E Protein, active, 250 µg
OverviewComplex of recombinant full length human cdk3 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive complex of recombinant full length human Cdk3 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceBoth are expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0.
Gene Symbol
  • CDK3
Protein TargetCDK3/cyclinE
Purification MethodNi-NTA agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q00526 # Probably involved in the control of the cell cycle. Interacts with a yet unknown type of cyclin. Can phosphorylate histone H1.
SIZE: 305 amino acids; 35046 Da
SUBUNIT: Interacts with CABLES1 (By similarity).
SIMILARITY: SwissProt: Q00526 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight36kDa (cdk3), 74kDa (cyclin E)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-487) and in bulk (cat# 14-487-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-487M 04053252269479

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-487 Cdk3/Cyclin E Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases