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218769 Caspase-8, Human, Recombinant, E. coli

Caspase-8, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FLICE, MACH, Mch5

218769
  
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Overview

Replacement Information
Description
OverviewRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
M.W. 11,000 and 18,000 (heterodimer).
Catalogue Number218769
Brand Family Calbiochem®
SynonymsFLICE, MACH, Mch5
References
ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Boldin, M.P., et al. 1996. Cell 85, 803.
Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
Muzio, M., et al. 1996. Cell 85, 817.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 30°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218769 0

Documentation

Caspase-8, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Caspase-8, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218769

References

Reference overview
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Boldin, M.P., et al. 1996. Cell 85, 803.
Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
Muzio, M., et al. 1996. Cell 85, 817.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-September-2007 RFH
    SynonymsFLICE, MACH, Mch5
    DescriptionRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
    FormLiquid
    FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
    Purity≥90% by SDS-PAGE
    Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (Cat. No. 368057) per min at 30°C, pH 7.4.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
    Toxicity Standard Handling
    ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
    Boldin, M.P., et al. 1996. Cell 85, 803.
    Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
    Muzio, M., et al. 1996. Cell 85, 817.
    Citation
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
    		•