Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

218704 Casein Kinase II, α-Subunit, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhCKIIα, Protein Kinase CKIIα

218704
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
Note: 1 mU = 1 milliunit.
Catalogue Number218704
Brand Family Calbiochem®
SynonymsrhCKIIα, Protein Kinase CKIIα
References
ReferencesAllende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
FormLiquid
FormulationIn 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥1.0 unit/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Contaminantsslight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218704 0

Documentation

Casein Kinase II, α-Subunit, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Casein Kinase II, α-Subunit, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218704

References

Reference overview
Allende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-May-2008 JSW
SynonymsrhCKIIα, Protein Kinase CKIIα
DescriptionRecombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where the autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt sensitive with respect to its activity. Easily inactivated by treatment with heat, trypsin digestion, or urea.
FormLiquid
FormulationIn 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Contaminantsslight autoproteolysis yields one additional band of 39 kDa; however, no loss in activity is associated with the appearance of this 39 kDa product
Specific activity≥1.0 unit/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesAllende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.