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2520 Blot Restore Membrane Rejuvination Kit, 10x

Blot Restore Membrane Rejuvination Kit, 10x MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting
2520
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
WB
Description
Catalogue Number2520
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionBlot Restore Membrane Rejuvination Kit, 10x
OverviewThe Blot Restore kit is an innovative tool for studies involving Western blot analysis.

It is widely believed that antigen-containing membranes used for Western blot experiments should never be dried. However, this is not true. The Blot Restore kit allows multiple re-use of membranes that were developed with substrate and subsequently dried. In model experiments both PVDF and nitrocellulose membranes showed excellent performance after having been dried for at least two months.

Kit contains two components. Solutioin A re-hydrates dry membranes and washes out chemiluminescent developing reagents. Solution B strips both primary and secondary (labeled) antibodies off the membrane. Treated membranes are ready for blocking and subsequent re-probing with antibodies of choice.

Use of the Blot Restore Kit offers several advantages:

1) Conservation of samples that are expensive or available only in limited quantities,

2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,

3) Re-analysis of anomalous results and confirmation with the same or a different antibody,

4) Correcting errors in incubation with the wrong antibody,

5) Cost savings in reagents and time by reusing the same blot.
Materials Required but Not Delivered· Standard Blot or blot strips, on nitrocellulose or PVDF/nylon membrane.

· Blocking Solutions.

· Distilled Water, for reagent dilution.

· Plastic Trays for incubation of blots or blot strips during hydration, stripping, washing and blocking solutions.
References
Product Information
Components
  • Blot Restore Solution A (10x) - (1 container, 50 mL).
  • Blot Restore Solution B (10x) - (1 container, 50 mL).
Detection methodChemiluminescence
HS Code3822 19 90
Applications
Key Applications
  • Western Blotting
Application NotesThe Blot Restore Kit is effective for hydrating dry membranes and removing antibodies from Western blots that have been developed with chemiluminescence or color substrates. The Blot Restore solution will not remove colored precipitate from blots developed using colorimetric substrates.

The Blot Restore Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

This product is for research use only; not for diagnostic or in vivo use.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsKit components should be stored at 2-8° C upon arrival. Product is stable for 3 to 6 months after receipt.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
2520 04053252618208