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69968 Biotinylated T7•Tag® Monoclonal Antibody

69968
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
69968-3 Plastic ampoule 125 ul
Description
Overview

This product has been discontinued.





When used with Novagen's Streptavidin Alkaline Phosphatase Conjugate, Biotinylated T7•Tag Antibody enables a 5-fold increase in detection sensitivity of target proteins on Western blots compared to an anti-IgG conjugate strategy. This form of the antibody also enables specific detection in the presence of other mouse IgGs.

The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

This antibody is also available as a direct conjugate with alkaline phosphatase or horseradish peroxidase.

SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/IsotypeMouse monoclonal IgG2b
Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (developed with chemiluminescent substrates)
FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution1:5,000–1:10,000
Catalogue Number69968
Brand Family Novagen®
Product Information
Components
Quality LevelMQ100
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Global Trade Item Number
Catalogue Number GTIN
69968-3 04055977274080