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L6715-BC Biocoll 1.077

L6715-BC
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
L6715 500 ml
Description
OverviewBiocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. Densities of up to 1.1 g/ml can be adjusted using this hydrophilic polymer. For optimal pH and osmolality, adjusting Biocoll with an acid, preferably amidotrizoeic acid(ATA), and sodium hydroxide is required. Biocoll with densities of 1.077 g/ml (Cat. No. L6113/5) and 1.090 g/ml (Cat. No. L6125) are already adjusted accordingly.

Specifications:

Sterility: Pass
Functional Test: Pass
pH: 7.0-7.5
Osmolality: 0.290-0.320 Osm/kg H20
Endotoxin: < 1.0 EU/ml
Density: 1.076-1.078 g/ml
Color: Colorless
Appearance: Clear
Separating solutions

Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.


Recommendations:

The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. But you can also adjust a required density yourself diluting Easycoll correspondingly.
Table 36: Instruction how to dilute the stock solution of Biocoll (density 1.090 g/ml; cat. no. L 6125) with PBS (cat. no. L 1825/0)
All amounts given for a temperature of +20°C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions will influence agglomeration and increase formation of cell clusters.

Separating lymphocytes using Biocoll:

1. Biocoll separating solution (D = 1.077 at +20°C) is given to 15 or 25 ml centrifugal tubes in volumes of 7 ml and 10 ml, resp.
2. Equal parts of heparinized whole blood (50 U/ml heparin, stabilizer-free) and culture medium are mixed and carefully applied over Biocoll separating solution.
3. Centrifuge 1200xg for 20 min.
4. Take layer of enriched (70 - 100 %) lymphocytes (between plasma and Biocoll) with a Pasteur′s pipette and wash twice in culture medium:
• for 10 min at 300xg
• for 10 min at 200xg
5. Cell count as usual.
Catalogue NumberL6715-BC
Brand Family Calbiochem®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
L6715 04027269841362

Documentation