Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCT247 BioTracker MultiTASQ G-Quadruplex (G4) Cell Probe

The BioTracker MultiTASQ G-quadruplex G4 cell probe is optimized for cellular detection of G-quadruplexes. The MultiTASQ probe is a uniquely designed, versatile tool for nucleic acid (RNA, DNA) G4 te

 More>>
SCT247
1 mg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCT247
DescriptionBioTracker MultiTASQ G-Quadruplex (G4) Cell Probe
Alternate Names
  • G4 probe
  • G quadruplex probe
  • Live Cell Imaging Probe
  • G4 ligand
  • G4 tetrad probe
  • click chemistry probe
Background InformationG-quadruplexes are four-stranded structures consisting of stacked G tetrads (G4) formed within select guanine-rich nucleic acid sequences. G-quadruplexes are enriched in open chromatin regions but are also present in the nucleolus and cytoplasm. The formation of G4 structures is dynamic and cell-type specific, suggesting interaction with transcription factors and other proteins. G-quadruplexes have therefore attracted acute interest in their possible contribution in transcriptional regulation.

MultiTASQ was designed utilizing the template-assembled synthetic G-quartets (TASQ) concept as biomimetic ligand for G-quadruplexes, demonstrating high specificity for G4. MultiTASQ is water-soluble and exhibits a high degree of intracellular accessibility. MultiTASQ detects ribosomal RNA-G4 associated with perinuclear rough ER regions to a greater degree compared to BG4 antibody. MultiTASQ is an alkynylated probe that allows for background-free detection via click chemistry and is suitable for G4 capture chemi-precipitation and click imaging applications.

References
1. Nat. Chem. 2021, 13(7): 626-633
2. ACS Chem Biol. 2021, 16(5): 905-914
Product Information
Detection methodFluorescence
FormatLyophilized solid
PresentationLyophilized. White solid.
Quality LevelMQ100
Applications
ApplicationThe BioTracker MultiTASQ G-quadruplex G4 cell probe is optimized for cellular detection of G-quadruplexes. The MultiTASQ probe is a uniquely designed, versatile tool for nucleic acid (RNA, DNA) G4 tetrad detection and analysis. MultiTASQ is compatible with live-cell detection by microscopy and other fluorescent detection techniques. This probe is biotinylated to enable detection with fluorescent streptavidiin.
Key Applications
  • RNA Detection
  • DNA-binding Proteins
  • Nucleic Acid Purification and Concentration
Application NotesFluorescent detection of nucleic acid G4 quadruplexes in cells and tissues using click chemistry
Biological Information
Molecular Weight1800 g/mol
Product Usage Statements
Quality Assurance
Purity: ≥ 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ BioCyTASQ G-quadruplex G4 cell probe at -20°C, desiccated and protected from light
Packaging Information
Material Size1 mg
Global Trade Item Number
Catalogue Number GTIN
SCT247 04065268798799