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SCT282 BioTracker™ Mito Membrane Potential Live Cell Detection Kit

This live cell probe is highly sensitive to changes in mitochondrial membrane potential.

SCT282
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT282
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DescriptionBioTracker™ Mito Membrane Potential Live Cell Detection Kit
OverviewThe SCT282 Mito Membrane Potential Live Cell Detection Kit overcomes the limitations of reagents like
JC-1 dye, TMRE, and TMRM widely used to monitor mitochondrial embrane potential (MMP).
These existing reagents often exhibit low photostability and poor retention after aldehyde fixation. Such limitations result in poor reproducibility of experiments. SCT282 demonstrates remarkable retention and photostability that enable reliable, reproducible monitoring of MMP.
Alternate Names
  • mitochondrial membrane potential probe
  • MMP live cell probe
Background InformationMitochondria are important organelles that utilize oxygen to synthesize ATP, producing the necessary energy for living cells to function and survive. Decreased mitochondrial activity and mitochondrial dysfunction are associated with cancer, aging, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases (AD and PD). Mitochondrial membrane potential (MMP) has therefore been widely studied in the context of health and disease where mitochondrial function is a key indicator.

References
1) K. F. Ferri, et al., J. Exp. Med., 2000, 192, 1081.
2) N. Matsuda, et al., J. Cell Biol., 2010, 189, 211.
3) J. L. Wang, et al., PNAS, 2000, 97, 7124.
References
Product Information
Detection methodFluorescence
FormatLiquid Reagent
Presentationkit containing liquid reagents
Quality LevelMQ100
Applications
ApplicationThis live cell probe is highly sensitive to changes in mitochondrial membrane potential.
Key Applications
  • Cell Based Assays
  • Cell Culture
  • Flow Cytometry
  • Fluorescence
Application NotesSCT282 is a live cell probe, but is also amenable to fixation after staining.
Diluting BioTracker™ Mito Membrane Potential Live Cell Detection Kit 1:1000 is recommended, but dilution may vary from 1:500 to 1:2000, depending on the end user application.
Dilute the 10x Imaging Buffer (1:10) in double-deionized water.
Dilute the MT-1 Dye (1:1000) in the cell culture medium to prepare working solution, and use the same day.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ Mito Membrane Potential Live Cell Detection Kit at -20 °C, protected from light.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT282 04065269218609

Documentation