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SCT037 BioTracker Far-red Labile Fe2+ Dye

Live Cell imaging probe for labile ferrous (Fe2+) ions, localizing inside endoplasmic reticulum (ER). Also suitable for flow cytometry.

SCT037
5 x 50 nmol  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCT037
DescriptionBioTracker Far-red Labile Fe2+ Dye
OverviewIron, the most abundant transition metal in our bodies, is involved in a number of biologically important processes such as respiration, oxygen transport, and energy production in collaboration with oxygen. In living cells, iron exists mainly as ferrous (Fe2+) and ferric (Fe3+) ionic forms. As a major contributor to oxidative damage of cells, Fe2+ is implicated in serious diseases such as cancers and neurodegenerative disorders, because of its ability to produce harmful reactive oxygen species via contact with oxygen, superoxide, and hydrogen peroxide (H2O2).

BioTracker far-red labile Fe2+ dye is a fluorescent probe that specifically detects labile iron (II) ions (Fe2+). This probe is designed to selectively react with only Fe2+ separately from other metal ions and irreversibly turns into a far-red fluorescent substance. It mainly localizes inside endoplasmic reticulum (ER) and/or golgi bodies. It can also be used with a flow cytometer equipped with a red laser. FerroFarRed shows almost no cytotoxicity at concentrations up to 100 μM (~20 times that of generally used concentrations).

This product is same as FerroFarRedTM (GC903-01) from Goryo Chemical, Inc.

Spectral Properties
Absorbance (max): 646nm
Emission (max): 662nm (Far-red)

References
Hirayama T et al. A universal fluorogenic switch for Fe (II) ion based on N-oxide chemistry permits the visualization of intracellular redox equilibrium shift towards labile iron in hypoxic tumor cells. Chem Sci. 2017. Jul 1;8(7): 4858-4866.
Alternate Names
  • FerroFarRed™
Product Information
Detection methodFluorescence
PresentationLyophilized. Solid Blue.
Applications
ApplicationLive Cell imaging probe for labile ferrous (Fe2+) ions, localizing inside endoplasmic reticulum (ER). Also suitable for flow cytometry.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging. Far-red fluorescence.
Product Usage Statements
Quality AssurancePurity: ≥ 95% (LC)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker Far-red Labile Fe2+ Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size5 x 50 nmol
Global Trade Item Number
Catalogue Number GTIN
SCT037 04061841588238