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AB7356 Anti-von Willebrand Factor Antibody

AB7356
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RELISA, IH(P), WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB7356
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-von Willebrand Factor Antibody
Alternate Names
  • Factor VIII Related Antigen
Background Informationvon Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.
Product Information
FormatPurified
PresentationPurified rabbit immunoglobulin in buffer containing 0.02 M PBS pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-von Willebrand Factor, Cat. No. AB7356, is a rabbit polyclonal antibody that detects von Willebrand Factor and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected von Willebrand Factor in Immunohistochemistry applications.

ELISA Analysis: A representative lot detected von Willebrand Factor in ELISA applications.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected von Willebrand Factor in human cerebral cortex, mouse hippocampus, rat cerebellum and human tonsil tissue sections.
Biological Information
ImmunogenHuman von Willebrand Factor purified from plasma.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityAB7356 is specific for vWF by ELISA against the human protein and by immunohistochemistry. The antibody is reactive in paraffin embedded tissues on human, rat, and mouse. Other species not tested. Antibody has not been evaluated by western blot analysis for reactivity to vWF.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.
Gene Symbol
  • VWF
  • F8VWF
  • F8
  • vWF
  • VWD
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04275 # Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
SIZE: 2813 amino acids; 309299 Da
SUBUNIT: Multimeric. Interacts with F8.
SUBCELLULAR LOCATION: Secreted. Note=Localized to storage granules.
TISSUE SPECIFICITY: Plasma.
DOMAIN: SwissProt: P04275 The von Willebrand antigen 2 is required for multimerization of VWF and for its targeting to storage granules.
PTM: All cysteine residues are involved in intrachain or interchain disulfide bonds.
DISEASE: SwissProt: P04275 # Defects in VWF are associated with various forms of von Willebrand disease (VWD) [MIM:193400, 277480]. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.). Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF. There are subtypes (A to H) of type II VWD; for example: type IIA is characterized by the absence of VWF high molecular weight multimers in plasma.
SIMILARITY: Contains 1 CTCK (C-terminal cystine knot-like) domain. & Contains 4 TIL (trypsin inhibitory-like) domains. & Contains 3 VWFA domains. & Contains 3 VWFC domains. & Contains 4 VWFD domains.
Molecular Weight~250 kDa observed; 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceImmunohistochemistry(paraffin):
Representative images from a previous lot.
Rabbit anti-vWF (AB7356) staining of Human Cerebral Cortex. Tissue pretreated with Citrate Buffer, ph 6.0, anti-vWF at 20 μg/mL, IHC-Select Detection with HRP-DAB.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
AB7356 04053252506710