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ABS2234-25UG Anti-phospho-PKLR (Ser12)

Anti-phospho-PKLR (Ser12), Cat. No. ABS2234, is a rabbit polyclonal antibody that detects Pyruvate kinase PKLR phosphorylated on serine 12 and is tested for use in Western Blotting.

ABS2234-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2234-25UG
DescriptionAnti-phospho-PKLR (Ser12)
Alternate Names
  • Pyruvate kinase PKLR
  • EC:2.7.1.40
  • L-PK
  • Pyruvate kinase isozymes L
Background InformationPyruvate kinase PKLR (UniProt: P12928-2; also known as EC:2.7.1.40, L-PK, Pyruvate kinase isozymes L/R) is encoded by the Pklr gene (Gene ID: 24651) in rat. Pyruvate kinas (PK) is a key glycolytic enzyme that catalyzes the hydrolysis of phosphoenolpyruvate (PEP) to pyruvate, generating ATP in this process. Four tissue specific isozymes of PK have been described in mammals: L (liver), R (erythrocytes), M1 (muscle) and ubiquitous M2. Except for M1 isozyme all other isozymes display positive cooperative kinetic behavior upon binding the allosteric activator fructose-1,6-diphosphate and in the presence of increasing concentrations of PEP. Phosphorylation of liver PK at serine 12 by protein kinase A (PKA), in response to glucagon, is shown to inhibit its enzymatic activity. Phosphorylation at serine 12 increases the Km for PEP by disrupting an activating interaction between its N-terminal unstructured domain and the main body of the protein. Studies have shown a significant decline in liver pyruvate kinase activity in insulin resistance. Also, it has been reported that phospho-Ser 12 form is significantly reduced in high fat fed mice. However, rats fed a high fat diet, but treated with a pyruvate kinase activator, do not exhibit any significant difference in their phosphor-Ser 12 form. (Ref.: Abulizi, A., et al. (2020). Cell Metab. 32(5); 751-766; Gassaway, BM., et al. (2019). Cell Rep. 29(11); 3394-3404).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-phospho-PKLR (Ser12), Cat. No. ABS2234, is a rabbit polyclonal antibody that detects Pyruvate kinase PKLR phosphorylated on serine 12 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-PKLR (Ser12) Western Blotting application. (Courtesy of Dr. Jesse Rinehart Lab at Yale University).

Western Blotting Analysis: A representative lot detected phospho-PKLR (Ser12) in Western Blotting applications (Gassaway, B.M., et.al. (2019). Cell Rep. 29(11):3394-3404).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding the phosphoserine 12 from the N-terminal region of rat Pyruvate. kinase PKLR.
EpitopeN-terminal
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Pyruvate Kinase PKLR phosphorylated on serine 12.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Mouse, Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pklr
Modifications
  • Phosphorylation
Purification MethodProtein A purified
UniProt Number
Molecular Weight~58 kDa observed; 58.79 kDa calculated for L-isoform. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Full-length pyruvate kinase PKLR phosphorylated at serine 12.

Western Blotting Analysis: A A 1:1,000 dilution of this antibody detected full-length rat Pyruvate kinase (PKLR) phosphorylated on serine 12, but the unphosphorylated pyruvate kinase
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2234-25UG 04065265324366