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05-481 Anti-phospho-MAP Kinase1/2 Antibody

05-481
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Av, CaWB, IP, ELISA, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-481
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-MAP Kinase1/2 Antibody
Alternate Names
  • Mitogen-activated protein kinase 3
  • MAP kinase 3
  • MAPK3
  • ERT2
  • Extracellular signal-regulated kinase 1
  • ERK-1
  • Insulin-stimulated MAP2 kinase
  • MAP kinase isoform p44
  • p44-MAPK
  • Microtubule-associated protein 2 kinase
  • p44-ERK1
Background InformationMitogen-activated protein kinase 3 (UniProt: P27632; also known as EC:2.7.11.24, MAP kinase 3, MAPK 3. ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase p44-ERK1) is encoded by the MAPK3 (also known as ERK1, PRKM3) gene (Gene ID: 5595) in human. ERK-1 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is expressed in different tissues and its highest levels have been observed in the nervous system. The MAPK/ERK cascade mediates several diverse biological processes such as cell growth, adhesion, survival, and differentiation through the regulation of transcription, translation, and cytoskeletal rearrangements. It also plays a role in initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a few transcription factors. About 160 substrates have been described for ERKs. The protein kinase domain of ERK-1 is localized to amino acids 42-330 and the TXY motif is located in amino acids 202-204. ERK-1 is phosphorylated by MEK1 and MEK2 on threonine 202 and tyrosine 204 in response to external stimuli like insulin or nerve growth factor and both these phosphorylations are essential for its activity. Three isoforms of ERK-1 have been described that are produced by alternative splicing.
Product Information
FormatPurified
Control
  • Included Positive Control Lysate: Catalog # 12-302, EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg (20 μL) of reduced lysate per lane for minigels.
PresentationPurified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose.
Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.
Quality LevelMQ100
Applications
ApplicationDetect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA, IC & IHC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunoprecipitation: Use 1-10 μg per immuno-precipitation.
Biological Information
ImmunogenKLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4
Cloneclone 12D4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySpecific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Avian
  • Canine
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. ...
Gene Symbol
  • MAPK3
  • MGC20180
  • ERT2
  • HUMKER1A
  • p44-MAPK
  • PRKM3
  • ERK1
  • p44erk1
  • p44mapk
  • P44MAPK
  • HS44KDAP
  • p44-ERK1
  • P44ERK1
  • ERK-1
Modifications
  • Phosphorylation
Purification MethodThiophilic adsoption and size exclusion chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P27361 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 379 amino acids; 43136 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P27361 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ...
Molecular Weight42-44 kDa
Product Usage Statements
Quality AssuranceEvaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.

Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
05-481 04053252473951