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MABN1833 Anti-nAChR α4 Subunit Antibody, clone 369

Anti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.

Anti-nAChR α4 Subunit Antibody, clone 369 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRNA4, NACHRA4, NACRA4 UniProt Number: P43681
MABN1833
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Rb, MkIH(P), IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1833
DescriptionAnti-nAChR α4 Subunit Antibody, clone 369
Alternate Names
  • Neuronal acetylcholine receptor subunit alpha-4
  • alpha4 nAChR subunit
  • Cholinergic receptor, nicotinic, alpha polypeptide 4
  • nAChR alpha4 subunit
  • Neuronal nicotinic acetylcholine receptor alpha-4 subunit
Background InformationNeuronal acetylcholine receptor subunit alpha-4 (UniProt P43681; also known as alpha4 nAChR subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit) is encoded by the CHRNA4 (also known as NACRA4, NACHRA4) gene (Gene ID 1137) in human. The nicotinic acetylcholine (ACh) receptors (nAChRs) are activated by acetylcholine (ACh) and nicotinic agonists, such as cytisine, 1,1-dimethyl-4-phenylpiperazinium and carbachol. The nAChRs are ligand-gated pentameric cation channels. Currently, 12 neuronal and 5 muscle nAChR subunits have been identified. The nAChRs can exist as homopentamers of five identical alpha subunits (α7, α8, or α9), alpha heteropentamers composed of more than one type of alpha subunits (α7-α10), or as alpha/beta heteropentamers consisting of various combinations of alpha (α2-α6) and beta (β2-β4) subunits. The heteromeric alpha/beta types display high ligand affinity, but are insensitive to α-bungarotoxin (αBgt), while the alpha-only homomeric and heteromeric pentamers show lower agonist affinity, but high αBgt sensitivity. The α4β2 (two α4 and three β2 subunits) and α7 (five α7 subunits) nAChRs are the predominant nAChRs in the central nervous system (CNS) among mammalian species. The alpha-4 subunit is initially produced with a signal peptide (a.a. 1-28), the removal of which yields the mature 627-amino acid membrane protein with a large extracellular domain (a.a. 29-242), four transmembrane segments (a.a. 243-267, 275-293, 309-330, 601-619), and a large cytoplasmic loop (a.a. 331-600) between the third and fourth transmembrane domains.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: An 1:50 dilution from a representative lot detected nAChR α4 Subunit in human small intestine tissue.
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in monkey frozen retina sections by fluorescence immunohistochemistry (Liu, J., et al. (2009). Invest. Ophthalmol. Vis .Sci. 50(3):1408-1415).
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in various areas of the rabbit brainstem by fluorescence immunohistochemical staining of free-floating sections (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Immunofluorescence Analysis: A represenative lot detected an increase of nAChR α4-positive neurons in the A1, A2 and A6 brainstem noradrenergic areas of female rabbits 1 hr after mating by fluorescence immunohistochemical staining of free-floating sections. The number of nAChR α4-positive neurons subsided to basal level 2 hrs after mating (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Biological Information
ImmunogenRecombinant human nAChR α4 subunit.
Clone369
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rabbit
  • Monkey
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHRNA4
  • NACHRA4
  • NACRA4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight67.16 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected nAChR α4 Subunit in human cerebellum tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1833 04055977329537