Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE1075 Anti-mono- and poly-ADP-ribose binding reagent

Anti-mono- and poly-ADP-ribose binding reagent, Cat. No. MABE1075, is a reagent that targets mono- and poly-ADP-Ribosylated proteins and has been tested in Dot Blots and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function UniProt Number: N/A
MABE1075
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MDB, WBE. coliPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1075
DescriptionAnti-mono- and poly-ADP-ribose binding reagent
Background InformationAnti-mono and poly ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both poly-and mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
References
Product Information
FormatPurified
PresentationPurified E. coli recombinant monoclonal binding reagent in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM -Mercaptoethanol without preservatives with 10% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-mono- and poly-ADP-ribose binding reagent, Cat. No. MABE1075, is a reagent that targets mono- and poly-ADP-Ribosylated proteins and has been tested in Dot Blots and Western Blotting.
Key Applications
  • Dot Blot
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.4 µg/mL of a representative lot detected mono- and poly-ADP ribosylated proteins.

Dot Blot Analysis: A representative lot detected mono- and poly-ADP-ribosylated proteins.

Dot Blot Analysis: A representative lot detected mono- and poly-ADP-ribosylated proteins (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).

Western Blotting Analysis: A representative lot detected mono-and poly-ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Biological Information
ConcentrationPlease refer to lot specific datasheet.
HostE. coli
SpecificityThis reagent binds to both mono- and poly-ADP ribosylated proteins.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Modifications
  • ADP-ribosylation
Purification MethodImmobilized Metal Affinity Chromatography (IMAC)
UniProt Number
Molecular WeightVariable depending on the target proteins and the extent of ADP-ribosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Gel Electrophoresis.

Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1075 04054839330636