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MABE463 Anti-methylated TLS/FUS Antibody, clone 9G6

Anti-methylated TLS/FUS, clone 9G6, Cat. No. MABE463, is a highly specific rat monoclonal antibody that targets methylated RNA-binding protein FUS and has been tested in Western Blotting.

Anti-methylated TLS/FUS Antibody, clone 9G6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: FUS, TLS UniProt Number: P35637
MABE463
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE463
DescriptionAnti-methylated TLS/FUS Antibody, clone 9G6
Alternate Names
  • RNA-binding protein FUS
  • 75 kDa DNA-pairing protein
  • Oncogene FUS
  • Oncogene TLS
  • POMp75
  • Translocated in liposarcoma protein
Background InformationRNA-binding protein FUS (UniProt: also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Fused in sarcoma/translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It is involved in a variety of cellular functions including transcription, protein translation, RNA splicing, and transport and may play a role in maintenance of genomic integrity. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. (Ref.: Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-methylated TLS/FUS, clone 9G6, Cat. No. MABE463, is a highly specific rat monoclonal antibody that targets methylated RNA-binding protein FUS and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of HeLa cell lysate.

Western Blotting Analysis: 0.05 ug/mL frrom a representative lot detected methylated TLS/FUS in 10 µg of lysate from HeLa cells pretreated with AdOx. (Dormann, D., et al. (2012). The EMBO J. 31(22):4258-75)

Western Blotting Analysis: A representative lot detected methylated TLS/FUS in Western Blotting applications (Suárez-Calvet, M., et. al. (2016). Acta Neuropathol. 131(4):587-604).
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 31 amino acids from the C-terminal region corresponding to the RGG3 domain of FUS (with all arginines methylated).
Clone9G6
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 9G6 detects methylated RNA-binding protein FUS in human cells. It targets an epitope with in 31 amino acids from the C-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FUS
  • TLS
Modifications
  • Methylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~70 kDa observed; 53.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.05 ug/mL of this antibody detected methylated TLS/FUS in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE463 04054839250972