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ABN26-25UG Anti-iNOS/NOS II Antibody, NT (rabbit polyclonal)

Detect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P).

ABN26-25UG
25 μg  
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Description
Catalogue NumberABN26-25UG
DescriptionAnti-iNOS/NOS II Antibody, NT (rabbit polyclonal)
Alternate Names
  • Nitric oxide synthase, inducible
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • Macrophage NOS
  • MAC-NOS
  • NOS type II
  • Peptidyl-cysteine S-nitrosylase NOS2
Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
References
Product Information
FormatAffinity Purified
Control
  • IFNgamma/LPS untreated and treated RAW264.7 cell lysates
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P).
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: 1:50-200 dilution from a representative lot detected iNOS/NOS II in malignant human lung tissues.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated iNOS/NOSII from IFNgamma/LPS treated RAW264.7 cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes iNOS/NOS II at the N-terminus.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteDemonstrated to react with Mouse and Human. Other homologies: Rat (85% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nos2
  • Inosl
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

CATALYTIC ACTIVITY: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

COFACTOR: Heme group.

Binds 1 FAD.

Binds 1 FMN.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.

ENZYME REGULATION: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.

SUBUNIT STRUCTURE: Homodimer. Binds SLC9A3R1 (By similarity).

TISSUE SPECIFICTY: Macrophages.

INDUCTION: By treatment with endotoxins or cytokines.

SEQUENCE SIMILARITIES: Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.
Molecular Weight~125 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected iNOS/NOS II on 10 µg of IFNgamma/LPS untreated and treated RAW264.7 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN26-25UG 04054839342639

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