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MABF1098 Anti-hnRNP A2B1 Antibody, clone 12B12.2

This mouse monoclonal Anti-hnRNP A2B1, clone 12B12.2 Antibody, Cat. No. MABF1098 is tested in Immunohistochemistry (Paraffin) and in Western Blotting for the detection of hnRNP.

Anti-hnRNP A2B1 Antibody, clone 12B12.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: HNRNPA2B1 UniProt Number: P22626
MABF1098
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1098
DescriptionAnti-hnRNP A2B1 Antibody, clone 12B12.2
Alternate Names
  • Heterogeneous nuclear ribonucleoproteins A2/B1
Background InformationHeterogeneous nuclear ribonucleoproteins A2/B1 (UniProt: P22626; also known as hnRNP A2/B1) is encoded by the HNRNPA2B1 gene (Gene ID: 3181) in human. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. It contains 2 RNA recognition motifs. Through alternate splicing two isoforms are generated. Isoform 1 (B1) is localized in cytoplasmic mRNP granules containing untranslated mRNA and in exosomes. It is not found in the nucleolus. Isoform 2 (A2) is predominantly nucleoplasmic, however it is also found in the cytoplasm of cells in some tissues. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. The hnRNP proteins are involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons and they acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm. They are also reported to specifically bind single-stranded telomeric DNA sequences, thereby protecting telomeric DNA repeat against endonuclease digestion.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-hnRNP A2B1, clone 12B12.2 Antibody, Cat. No. MABF1098 is tested in Immunohistochemistry (Paraffin) and in Western Blotting for the detection of hnRNP.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative detected hnRNP A2B1 in human tonsil, human small intestine, and human uterus tissues.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected hnRNP A2B1 in 10 µg of NIH/3T3.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human heterogeneous nuclear ribonucleoproteins A2/B1.
Clone12B12.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 12B12.2 specifically detects heterogeneous nuclear ribonucleoproteins A2/B1. it targets an epitope within 15 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HNRNPA2B1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~37 kDa observed; 37.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected hnRNP A2B1 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1098 04054839189104