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OP30L Anti-c-Myc (Ab-2) Mouse mAb (8)

Anti-c-Myc (Ab-2) Mouse mAb (8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP30L
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~60-67 kDa c-Myc protein in HL60 cells.
Catalogue NumberOP30L
Brand Family Calbiochem®
References
ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
Nau, M.N., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Dalla-Favera, R., et al. 1983. Science 219, 963.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 1 mg/ml BSA.
Negative controlHT1080 cells
Positive controlHL-60 cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
Application CommentsThe c-myc protein is extremely labile. c-myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. The sequence of c-myc predicts a ~45 kDa protein, but c-myc migrates under reducing conditions as a ~64-67 kDa band. This is the preferred antibody for immunoblotting. For best results with immunoprecipitation, compare results with and without SDS. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human c-myc
ImmunogenHuman
Clone8
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP30L 0

Documentation

Anti-c-Myc (Ab-2) Mouse mAb (8) Certificates of Analysis

TitleLot Number
OP30L

References

Reference overview
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
Nau, M.N., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Dalla-Favera, R., et al. 1983. Science 219, 963.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-February-2008 JSW
ApplicationImmunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclnal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells. Recognizes the ~60-67 kDa (apparent MW) c-Myc protein.
BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. In both mouse plasmacytomas and human Burkitt's lymphoma, coding sequences from the c-myc gene are translocated from their normal chromosomal position into a transcriptionally active immunoglobulin locus on another chromosome. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Furthermore, elevated levels of myc mRNA and protein have been demonstrated in human tumors even in the absence of gene amplification. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human c-myc
Clone8
IsotypeIgG2a
Speciesavian, human, mouse
Positive controlHL-60 cells
Negative controlHT1080 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 1 mg/ml BSA.
PreservativeNone
CommentsThe c-myc protein is extremely labile. c-myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. The sequence of c-myc predicts a ~45 kDa protein, but c-myc migrates under reducing conditions as a ~64-67 kDa band. This is the preferred antibody for immunoblotting. For best results with immunoprecipitation, compare results with and without SDS. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
Nau, M.N., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Dalla-Favera, R., et al. 1983. Science 219, 963.