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07-329-AF488 Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor® 488

This Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor® 488 is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4 (Lys16).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HHF1, YBR009C, YBR0122, HHF2, YNL030W, N2752 UniProt Number: P02309
07-329-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number07-329-AF488
DescriptionAnti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor® 488
Alternate Names
  • Histone H4
  • acetyl-Histone H4 (Lys16), AlexaFluor® 488
Background InformationHistone H4 (UniProt P02309) is encoded by the HHF1 (ORF YBR0122; Gene ID 852294) and HHF2 (ORF N2752; Gene ID 855701) genes in yeast species. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor® 488 is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4 (Lys16).
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 (Lys16) in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.
Biological Information
ImmunogenOvalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
EpitopeH4K16ac
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityH4K16ac specificity of the unconjugated antibody (Cat. No. 07-329) has been confirmed using the Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology. Broad species cross-reactivity is expected.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HHF1
  • YBR009C
  • YBR0122
  • HHF2
  • YNL030W
  • N2752
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~10 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
07-329-AF488 04055977300826