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06-942-AF647 Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 647 Conjugate

Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate Antibody, Cat. No. 06-942-AF647, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has bee

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Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: Q16695
06-942-AF647
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICC, FCRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue Number06-942-AF647
DescriptionAnti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • Lys9-acetylated
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate Antibody, Cat. No. 06-942-AF647, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.
Key Applications
  • Immunocytochemistry
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.

The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
Biological Information
ImmunogenKLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityHistone H3 acetylated at position 9.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Modifications
  • Acetylation
Purification MethodProtein A purified.
UniProt Number
Molecular Weight~17 kDa observed.
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys9 acetylation (H3K9ac) in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
06-942-AF647 04054839056215