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ABN2242-25UG Anti-Vangl2

Anti-Vangl2, Cat. No. ABN2242, is a highly specific rabbit polyclonal antibody that targets Vang-like protein 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Vangl2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: VANGL2, KIAA1215, STB1 UniProt Number: Q9ULK5
ABN2242-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HICC, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN2242-25UG
DescriptionAnti-Vangl2
Alternate Names
  • Vang-like protein 2 (UniProt: Q9ULK5
  • also known as Loop-tail protein 1 homolog
  • Strabismus 1
  • Van Gogh-like protein 2
Background InformationVang-like protein 2 (UniProt: Q9ULK5; also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2) is encoded by the VANGL2 (also known as KIAA1215, STB1) gene (Gene ID: 57216) in human. Vangl2 is a homodimeric multi-pass membrane protein that plays a role in the control of early morphogenesis, patterning of both axial midline structures and the development of neural plate. It can also form heterodimer with VANGL1. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Vang-like protein 2 is reported to bind to Dvl to cell-autonomously promote efficient Dvl plasma membrane recruitment, a pre-requisite for planar cell polarity activation. At the same time, it can inhibit Dvl from interacting with its downstream effector (Dishevelled associated activator of morphogenesis 1 (Daam1), and functionally suppresses Dvl - Daam1 cascade. Mutations in VANGL2 gene cause neural tube defects that results in defective neural tube closure during the first trimester of pregnancy. (Ref.: Seo, HS et al. (2017). Hum. Mol. Genet. 26(11); 2053-2061).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Vangl2, Cat. No. ABN2242, is a highly specific rabbit polyclonal antibody that targets Vang-like protein 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vangl2 in HT1080 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Vangl2 in human tonsil and mouse embryonic spinal cord tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Vang-like Protein 2.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Vang-like protein 2 in human and murine cells. It targets an epitope with in 15 amino acids from the N-terminal region.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman and Mouse. Predicted to react with Bovine and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VANGL2
  • KIAA1215
  • STB1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Vangl2 in HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN2242-25UG 04054839448188