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PC322L Anti-VEGFR1 Goat pAb

This Anti-VEGFR1 Goat pAb is validated for use in Competition Studies, ELISA, Immunoblotting, Paraffin Sections for the detection of VEGFR1.

Anti-VEGFR1 Goat pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor

PC322L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HGtPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes VEGF receptor 1.
Catalogue NumberPC322L
Brand Family Calbiochem®
SynonymsAnti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor
References
ReferencesSoker, S., et al. 1998. Cell 92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
Kendall, R.L. and Thomas, K.A. 1993. PNAS. 90, 10705.
Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
Brown, L., et al. 1992. Kidney International 42, 1457.
Product Information
FormLyophilized
FormulationLyophilized from a sterile solution in PBS, 5% trehalose.
Positive controlEndothelial cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Competition Studies
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesCompetition Studies (see comments)
ELISA (0.5-1 µg/ml)
Immunoblotting (0.1-0.2 µg/ml)
Paraffin Sections (5-15 µg/ml)
Application CommentsThis antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human VEGFR1 (extracellular domain)/Fc chimera
ImmunogenHuman
HostGoat
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC322L 0

Documentation

Anti-VEGFR1 Goat pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-VEGFR1 Goat pAb Certificates of Analysis

TitleLot Number
PC322L

References

Reference overview
Soker, S., et al. 1998. Cell 92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
Kendall, R.L. and Thomas, K.A. 1993. PNAS. 90, 10705.
Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
Brown, L., et al. 1992. Kidney International 42, 1457.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-July-2007 RFH
SynonymsAnti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor
ApplicationCompetition Studies (see comments)
ELISA (0.5-1 µg/ml)
Immunoblotting (0.1-0.2 µg/ml)
Paraffin Sections (5-15 µg/ml)
DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the VEGF receptor 1 protein.
BackgroundVascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a secreted heparin binding cytokine expressed in tumor and animal cells. VEGF has been implicated in the control of angiogenesis due to its selective mitogenic stimulation of vascular endothelial cells and enhancement of vascular permeability. VEGF is a ~34-50 kDa dimer of two identical disulfide-linked subunits existing in four molecular species; 121, 165, 189, and 206 amino acid residues long, arising from alternative splicing of a single gene. VEGF binds to two receptor tyrosine kinases, Flt-1 (VEGFR1) and KDR/Flk-1 (VEGFR2) found almost exclusively on human endothelial cells. Flt-1 binds VEGF and a new VEGF-related ligand, placenta growth factor (PIGF) but KDR/Flk-1 binds only VEGF. The cDNA encoding the soluble truncated form of Flt-1 cloned from a human endothelial cell library encodes the 6 N-terminal IgG-like extracellular ligand-binding domain but does not encode the transmembrane spanning region and intracellular tyrosine kinase domains. The recombinant soluble receptor binds avidly to VEGF and is a potent VEGF antagonist. Recently, a third VEGF receptor was identified and cloned from tumor cells. This VEGF receptor is isoform-specific, binding VEGF165 but not VEGF121. Studies indicate that this VEGF receptor (VEGF165R) is identical to human neuropilin-1, a receptor for the collapsin/semaphorin family that mediates neuronal cell guidance.
HostGoat
Immunogen speciesHuman
Immunogenrecombinant, human VEGFR1 (extracellular domain)/Fc chimera
IsotypeIgG
Specieshuman
Positive controlEndothelial cells
FormLyophilized
FormulationLyophilized from a sterile solution in PBS, 5% trehalose.
PreservativeNone
CommentsThis antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesSoker, S., et al. 1998. Cell 92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
Kendall, R.L. and Thomas, K.A. 1993. PNAS. 90, 10705.
Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
Brown, L., et al. 1992. Kidney International 42, 1457.