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BA24 Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1)

This Anti-Thrombospondin Mouse mAb is validated for use in Electron Microscopy, Neutralization Studies, FC, Frozen Sections, WB, Paraffin Sections for the detection of Thrombospondin.

Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TSP-1, Anti-TSP

BA24
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Eq, H, Porcine, R, ShMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
BA24-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~450 kDa (non-reducing conditions) and the ~180 kDa (reducing conditions) thrombospondin protein, in endothelial cells, fibroblasts, platelets, monocytes, macrophages, and tonsil tissue. Does not cross-react with fibronectin, fibrinogen, or von Willebrand factor.
Catalogue NumberBA24
Brand Family Calbiochem®
SynonymsAnti-TSP-1, Anti-TSP
Application Data
Detection of human thombospondin by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1:50). Detection: fluorescence.

Detection of human Thrombospondin by immunoblotting. Sample: Human thrombospondin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesDawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
Jaffe, E.A., et al. 1983. PNAS 80, 998.
Saglio, S. and Slayter, H. 1982. Blood 59, 162.
Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
Product Information
FormLiquid
FormulationIn 10 mM PBS, pH 7.4.
Positive controlEndothelial cells, fibroblasts, platelets, monocytes, or macrophages in tonsil tissue
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesNeutralization Studies Galvin, N.J., et al. 1985. J. Cell Biol. 101, 1434.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Not Paraffin Sections
Neutralization Studies
Application NotesElectron Microscopy (see comments)
Neutralization Studies (see comments and application references)
Flow Cytometry (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunofluorescence (see comments)
Paraffin Sections (not recommended)
Application CommentsDoes not cross-react with fibronectin, fibrinogen, or von Willebrand factor. This antibody will inhibit TSP-collagen interactions. Its binding to TSP is unaffected by glycosaminoglycans (e.g., hyaluronic acid, chrondroitin sulfate and heparin). EDTA (i.e., low concentrations of Ca2+) enhances the binding capacity of Clone A6.1 to TSP. This antibody has been reported to work in immunofluorescence, flow cytometry, and electron microscopy. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenreduced and alkylated human thrombospondin
ImmunogenHuman
CloneA6.1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Canine
  • Horse
  • Human
  • Porcine
  • Rat
  • Sheep
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
BA24-100UG 04055977220735

Documentation

Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) Certificates of Analysis

TitleLot Number
BA24

References

Reference overview
Dawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
Jaffe, E.A., et al. 1983. PNAS 80, 998.
Saglio, S. and Slayter, H. 1982. Blood 59, 162.
Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-February-2009 JSW
SynonymsAnti-TSP-1, Anti-TSP
ApplicationElectron Microscopy (see comments)
Neutralization Studies (see comments and application references)
Flow Cytometry (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunofluorescence (see comments)
Paraffin Sections (not recommended)
Application Data
Detection of human thombospondin by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1:50). Detection: fluorescence.

Detection of human Thrombospondin by immunoblotting. Sample: Human thrombospondin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/Ag14 mouse myeloma cells. Recognizes the ~450 kDa (non-reduced) or the ~180 kDa (reduced) thrombospondin (TSP) protein.
BackgroundThrombospondin (TSP) is a ~450 kDa glycoprotein synthesized by platelets, as well as by monocytes, macrophages, fibroblasts and endothelial cells. Although TSP is the major α-granule protein of human platelets, the concentration of the protein in circulating plasma is quite low. Upon stimulation of platelets, the TSP is expressed on the cell surface where it plays a role in platelet aggregation and other cell-cell interactions. Studies show that the platelet-monocyte association has been demonstrated to be mediated by the interaction of TSP on the platelet surface with a monocyte TSP receptor. The receptor has been identified immunologically as glycoprotein IV, also known as GPIIIb and CD36, a ~90 kDa membrane glycoprotein expressed on platelets, endothelial cells, erythrocytes, monocytes, and some human tumor cells lines but not on lymphocytes or granulocytes. Recent studies have shown that TSP-1 is a naturally occurring inhibitor of angiogenesis whose actions are mediated by CD36, found on microvascular endothelial cells.
HostMouse
Immunogen speciesHuman
Immunogenreduced and alkylated human thrombospondin
CloneA6.1
IsotypeIgG₁
Speciesbovine, canine, horse, human, porcine, rat, sheep
Positive controlEndothelial cells, fibroblasts, platelets, monocytes, or macrophages in tonsil tissue
FormLiquid
FormulationIn 10 mM PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsDoes not cross-react with fibronectin, fibrinogen, or von Willebrand factor. This antibody will inhibit TSP-collagen interactions. Its binding to TSP is unaffected by glycosaminoglycans (e.g., hyaluronic acid, chrondroitin sulfate and heparin). EDTA (i.e., low concentrations of Ca2+) enhances the binding capacity of Clone A6.1 to TSP. This antibody has been reported to work in immunofluorescence, flow cytometry, and electron microscopy. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
Jaffe, E.A., et al. 1983. PNAS 80, 998.
Saglio, S. and Slayter, H. 1982. Blood 59, 162.
Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
Application referencesNeutralization Studies Galvin, N.J., et al. 1985. J. Cell Biol. 101, 1434.