Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABN2266-25UL Anti-Tentonin 3/TMEM150c

Anti-Tentonin 3/TMEM150c, Cat. No. ABN2266, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 150C and has been tested in , Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

ABN2266-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABN2266-25UL
DescriptionAnti-Tentonin 3/TMEM150c
Alternate Names
  • Transmembrane protein 150C
  • TTN3
  • Tentonin 3
Background InformationTransmembrane protein 150C (UniProt: Q8C8S3; also known as Tentonin, TTN3) is encoded by the Tmem150c gene (Gene ID: 231503) in murine species. TTN3 is a multi-pass membrane protein of the DRAM/TMEM150 family. Two isoforms of TTN3 have been described that are produced by alternative splicing. It belongs to a new class of mechanotransduction channel that is expressed in muscle spindle afferents and contribute to proprioception (touch sensation). It is activated by mechanical stimuli with distinctly slow inactivation kinetics. Mice with deficiency in TTN3 display loss of coordinated movements and abnormal gait. It is reported to mediate slow adapting currents in DRG neurons.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Tentonin 3/TMEM150c, Cat. No. ABN2266, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 150C and has been tested in , Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in muscle spindle from mouse (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).

Immunocytochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in HEK293T cells transfected with TTN3. (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).

Western Blotting Analysis: A representative lot detected Tentonin 3/TMEM150c in F11 rat DRG cells (untransfected and TTN3 transfected cells) (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).
Biological Information
ImmunogenA linear peptide corresponding to 38 amino acids from the C-terminal region of murine Transmembrane protein 150C.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Transmembrane protein 150C in mouse and rat cells. it targets an epitope within 38 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Tmem150c
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~25 kDa observed; 27.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in F11 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Tentonin 3/TMEM150c in 10 µg of F11 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN2266-25UL 04054839518638

Related Products & Applications