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574788 Anti-Syntaxin 4 (2-23) Rabbit pAb

This Anti-Syntaxin 4 (2-23) Rabbit pAb is validated for use in Immunoblotting for the detection of Syntaxin 4 (2-23).

Anti-Syntaxin 4 (2-23) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody
Description
OverviewRecognizes the syntaxin 4 protein. Supplied with a control peptide.
Catalogue Number574788
Brand Family Calbiochem®
References
ReferencesTellam, J.T., et al. 1997. J. Biol. Chem. 272, 6179.
Mandon, B., et al. 1996. J. Clin. Invest. 98, 906.
Bennett, M.K., et al. 1993. Cell 74, 863.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:200-1:500, chemiluminescence)
Application CommentsTo minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [RDRTHELRQGDNISDDEDEVRV(C)] corresponding to amino acids 2-23 of rat syntaxin 4, conjugated to carrier protein
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl deionized H₂O. Following reconstitution, aliquot and freeze (-20°C). Reconstitute control peptide with 100 µl dH₂O. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
574788 0

Documentation

Anti-Syntaxin 4 (2-23) Rabbit pAb Certificates of Analysis

TitleLot Number
574788

References

Reference overview
Tellam, J.T., et al. 1997. J. Biol. Chem. 272, 6179.
Mandon, B., et al. 1996. J. Clin. Invest. 98, 906.
Bennett, M.K., et al. 1993. Cell 74, 863.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-August-2008 RFH
ApplicationImmunoblotting (1:200-1:500, chemiluminescence)
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the syntaxin 4 protein. Supplied with a control peptide (immunogen).
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide [RDRTHELRQGDNISDDEDEVRV(C)] corresponding to amino acids 2-23 of rat syntaxin 4, conjugated to carrier protein
IsotypeIgG
Speciesmouse, rat
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
Preservative≤0.1% sodium azide
CommentsTo minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl deionized H₂O. Following reconstitution, aliquot and freeze (-20°C). Reconstitute control peptide with 100 µl dH₂O. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesTellam, J.T., et al. 1997. J. Biol. Chem. 272, 6179.
Mandon, B., et al. 1996. J. Clin. Invest. 98, 906.
Bennett, M.K., et al. 1993. Cell 74, 863.