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MABS2034-25UG Anti-SREBP-1 Antibody, clone 2A4

Anti-SREBP-1, clone 2A4, Cat. No. MABS2034, is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Western Blotting.

Anti-SREBP-1 Antibody, clone 2A4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SREBF1, BHLHD1, SREBP1 UniProt Number: P36956
MABS2034-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ht, H, CaWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2034-25UG
DescriptionAnti-SREBP-1 Antibody, clone 2A4
Alternate Names
  • Sterol regulatory element-binding protein 1
  • Class D basic helix-loop-helix protein 1
  • bHLHd1
  • Sterol regulatory element-binding transcription factor 1
Background InformationSterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human. SREBP-1 is a multi-pass membrane protein found on nuclear, endoplasmic reticulum, and Golgi membranes. It attaches to membranes via two hydrophobic sequences (aa 477-497 and 536-556). It is expressed in a wide variety of tissues, but is most abundant in liver and adrenal gland. Six isoforms of SREBP-1 have been described that are produced by alternative splicing. Isoform SREBP-1C predominates in liver, adrenal gland, and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Both isoforms are found in kidney, brain, white fat, and muscle. It serves as a transcriptional activator that is essential for lipid homeostasis and regulates transcription of the LDL receptor gene. It is also involved in fatty acid and cholesterol synthesis. It acts by binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') and displays dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). SREBP-1 has two cytoplasmic domains (aa 1-487 and 569-1147), two transmembrane domains (aa 88-508 and 548-568), and one lumenal domain (aa 509-547). In the precursor form, which has a hairpin orientation in the membrane both the N-terminal transcription factor domain and the C-terminal regulatory domain face the cytoplasm. Two separate site-specific proteolytic cleavages are required for release of the transcriptionally active N-terminal domain. These cleavages are carried out by two distinct proteases, called site-1 protease (S1P; aa 490-491) and site-2 protease (S2P; aa 530-531). SREBP-1 can be phosphorylated by AMPK at Ser402, which suppress its processing and nuclear translocation, and thereby repress target gene expression.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-SREBP-1, clone 2A4, Cat. No. MABS2034, is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Clone2A4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Hamster
  • Human
  • Canine
Species Reactivity NoteHamster, Canine, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SREBF1
  • BHLHD1
  • SREBP1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~135 kDa observed; 121.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SREBP-1 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2034-25UG 04054839563317