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MABD390 Anti-SPRY1 Antibody, clone 7E6.2

This mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.

Anti-SPRY1 Antibody, clone 7E6.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Gene Symbol: SPRY1 UniProt Number: O43609
MABD390
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABD390
DescriptionAnti-SPRY1 Antibody, clone 7E6.2
Alternate Names
  • Protein sprouty homolog 1
  • Spry-1
Background InformationProtein sprouty homolog 1 (UniProt O43609; also known as Spry-1) is encoded by the SPRY1 gene (Gene ID 10252) in human. Sprouty was originally identified in Drosophila as a negative regulator of fibroblast growth factor (FGF) signaling during tracheal development. There exist four mammalian homologs (SPRY1-4) that likewise mediate feedback inhibition against growth factor-/receptor tyrosine kinase- (RTK-) dependent Ras/mitogen-activated protein (MAP) kinase signaling pathway. Loss of function studies in mice show that SPRY1 and SPRY2 play critical roles during early development of multiple organ rudiments, including external genitalia, eye, kidney, inner ear, tooth, and cortical patterning in the brain. In addition, evidences indicate that SPRY1 plays an antiproliferative and tumor-suppressing role in cancers. SPRY1 downregulation is reported in various cancer types, including prostate, breast, and ovarian cancers, and SPRY1 expresion has been proposed as a marker of good prognosis in patients with renal cell carcinoma.
References
Product Information
FormatCulture Supernatant
PresentationMouse IgG1 hybridoma culture supernatant without azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPRY1 in 10 µg of SK-MEL-2 human skin melanoma cell lysate.
Biological Information
ImmunogenGST-tagged recombinant human SPRY1 N-terminal fragment.
Clone7E6.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7E6.2 detects the ~35 kDa human SPRY1 by targeting an N-terminal region epitope.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SPRY1
Purification MethodUnpurified
UniProt Number
Molecular Weight~35 kDa observed. 35.12 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.


Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected SPRY1 in 10 µg of HepG2 human hepatocellular carcinoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABD390 04054839086205