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566310 Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb

This Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb is validated for use in Immunoblotting for the detection of SIRPα1, C-Terminal (487-503).

Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-BIT, Anti-MyD-1, Anti-p84, Anti-SHPS-1

566310
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~75-110 kDa SIRPα and SIRPα1 proteins in THP cells.
Catalogue Number566310
Brand Family Calbiochem®
SynonymsAnti-BIT, Anti-MyD-1, Anti-p84, Anti-SHPS-1
Application Data
Detection of human C-terminal SIRPα1 by immunoblotting. Sample: Whole cell lysate from THP-1 cells. Primary antibody: Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb (Cat. No. 566310) (1:2000). Detection: chemiluminescence.
References
ReferencesStofega, M.R., et al. 1998. J. Biol. Chem. 273, 7112.
Kharitonenkov, A., et al. 1997. Nature 386, 181.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlTHP-1 cells
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000-1:2000, colorimetric)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide [(C)KPEPSFSEYASVQVPRK] corresponding to amino acids 487-503 of human SIRPα1, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
566310 0

Documentation

Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb Certificates of Analysis

TitleLot Number
566310

References

Reference overview
Stofega, M.R., et al. 1998. J. Biol. Chem. 273, 7112.
Kharitonenkov, A., et al. 1997. Nature 386, 181.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2007 RFH
SynonymsAnti-BIT, Anti-MyD-1, Anti-p84, Anti-SHPS-1
ApplicationImmunoblotting (1:1000-1:2000, colorimetric)
Application Data
Detection of human C-terminal SIRPα1 by immunoblotting. Sample: Whole cell lysate from THP-1 cells. Primary antibody: Anti-SIRPα1, C-Terminal (487-503) Rabbit pAb (Cat. No. 566310) (1:2000). Detection: chemiluminescence.
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~75-110 kDa SIRPα and SIRPα1 proteins.
BackgroundSIRPα is a novel SHP-2-associated glycoprotein that is a substrate of many activated tyrosine kinases such as EGFR, insulin receptor, PDGFR, and Src. SIRPα has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones, and cell adhesion and plays a general role in other physiological and pathological processes.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide [(C)KPEPSFSEYASVQVPRK] corresponding to amino acids 487-503 of human SIRPα1, conjugated to KLH
IsotypeIgG
Specieshuman
Positive controlTHP-1 cells
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesStofega, M.R., et al. 1998. J. Biol. Chem. 273, 7112.
Kharitonenkov, A., et al. 1997. Nature 386, 181.