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CB1040 Anti-S100B Mouse mAb (1B2)

This Anti-S100B Mouse mAb (1B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of S100B.

Anti-S100B Mouse mAb (1B2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-S100 Calcium Binding Protein B

CB1040
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~10 kDa S100B protein in HepG2 cells and human salivary gland tissue.
Catalogue NumberCB1040
Brand Family Calbiochem®
SynonymsAnti-S100 Calcium Binding Protein B
Application Data
Detection of human S100B by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (1-5 µg/ml). Detection: chemiluminescences.

Detection of human S100B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (3 µg/ml). Detection: colorimentric (DAB)
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHepG2 cells, Human salivary gland tissue
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 1-93 human S100B, expressed as a GST fusion protein
Clone1B2
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1040 0

Documentation

Anti-S100B Mouse mAb (1B2) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-S100 Calcium Binding Protein B
ApplicationImmunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human S100B by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (1-5 µg/ml). Detection: chemiluminescences.

Detection of human S100B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-S100B Mouse mAb (1B2) (Cat. No. CB1040) (3 µg/ml). Detection: colorimentric (DAB)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~12 kDa S100B protein.
BackgroundThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 1-93 human S100B, expressed as a GST fusion protein
Clone1B2
IsotypeIgG₁
Specieshuman
Positive controlHepG2 cells, Human salivary gland tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review