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MABN2286-25UL Anti-Rai Antibody, clone IF15

Anti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

MABN2286-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2286-25UL
DescriptionAnti-Rai Antibody, clone IF15
Alternate Names
  • SHC-transforming protein 3
  • Neuronal Shc
  • N-Shc
  • Protein Rai
  • SHC-transforming protein C
  • Src homology 2 domain-containing-transforming protein C3
  • SH2 domain protein C3
Background InformationSHC-transforming protein 3 (UniProt: Q92529; also known as Neuronal Shc, N-Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3, SH2 domain protein C3, Rai) is encoded by the SHC3 (also known as NSHC, SHCC) gene (Gene ID:53358) in human. Rai protein is mainly expressed in the brain and its higher expression is observed in cerebral cortex, frontal and temporal lobes, hippocampus, and occipital pole. Its expression is also reported in the enteric glial cells in the human gut. It serves as a signaling adapter and is involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons. Rai also serves as a physiological substrate of the receptor tyrosine kinase Ret and increases Ret-dependent survival via PI3 kinase/Akt pathway activation. It also potentiates MAP kinase signaling. Rai activation also plays a role in long-term potential and is neuroprotective against certain ischemia and oxidative stress. Evolutionarily it correlates well with vertebrate brain development. Its reduced levels are observed in brain of Alzheimer s disease subjects. Two isoforms of Rai have been described (p64 and p52) that are produced by alternative splicing. (Ref.: Triaca, V., et al. (2018). Aging. 10(1); 5-6; Sagi, O., et al. (2015). Ageing Res. Rev. 19; 34-42; Villanacci, V., et al. (2008). Neurogastroenterol. Motil. 20(3); 206-212).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
Application NotesTested Applications

Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.

Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant fragment corresponding to 164 amino acids CH1 domain of human SHC-transforming protein 3 (Rai).
EpitopeC-terminal half
CloneIF15-E2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone IF15-E2 is a mouse monoclonal antibody that detects SHC-transforming protein 3/RAI. It targets an epitope within the CH1 domain from the C-terminal half.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SHC3
  • NSHC
  • SHCC
Purification MethodProtein G purified
UniProt Number
Molecular Weight~58 kDa observed; 64.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2286-25UL 04065266553901