Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABN1474-100UG Anti-RNF213

Anti-RNF213, Cat. No. ABN1474, is a highly specific rabbit polyclonal antibody that targets E3 ubiquitin-protein ligase RNF213 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-RNF213 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: RNF213, ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR UniProt Number: Q63HN8
ABN1474-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1474-100UG
DescriptionAnti-RNF213
Alternate Names
  • E3 ubiquitin-protein ligase RNF213
  • EC:2.3.2.27
  • ALK lymphoma oligomerization partner on chromosome 17
  • Mysterin
  • RING finger protein 213
  • RING-type E3 ubiquitin transferase RNF213
Background InformationE3 ubiquitin-protein ligase RNF213 (UniProt: Q63HN8; also known as EC:2.3.2.27, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF213) is encoded by the RNF213 (also known as ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR) gene (Gene ID: 57674) in human. RNF213 is a widely expressed, homooligomeric cytosolic protein that is involved in the non-canonical Wnt signaling pathway in vascular development. It acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, which leads to the inhibition of the non-canonical Wnt signaling pathway and promotes vessel regression. Its activity is induced by pro-inflammatory cytokines. RNF213 contains a Zinc finger domain (aa 3997-4036), which is required for its ubiquitin-protein ligase activity. It also contains an AAA domain, which is associated with ATPase activity. Four isoforms of RNF213 have been described that are produced by alternative splicing. Mutations in RNF213 gene have been linked to Moyamoya disease 2 (MYMY2) disease, a progressive cerebral angiopathy that can lead to transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-RNF213, Cat. No. ABN1474, is a highly specific rabbit polyclonal antibody that targets E3 ubiquitin-protein ligase RNF213 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RNF213 in human cerebral cortex and human kidney tissue sections.

Western Blotting Analysis: 2 µg/mL from a representative lot detected RNF213 in 10 µg of HEK293 cells transfected with 3XFLAG RNF213 WT.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase RNF213. Immunogen sequence is conserved in isoforms 1, 2, and 4.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects E3 ubiquitin-protein ligase RNF213 in human cells. It targets an epitope within 15 amino acids from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RNF213
  • ALO17
  • C17orf27
  • KIAA1554
  • KIAA1618
  • MYSTR
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~590 kDa observed; 591.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected RNF213 in 10 µg of HUVEC lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1474-100UG 04054839499456