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MABS1709-25UG Anti-R-Spondin 2 Antibody, clone 14C7.1

Anti-R-Spondin 2, clone 14C7.1, Cat. No. MABS1709, is a mouse monoclonal antibody that specifically targets R-Spondin 2 (RSPO2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-R-Spondin 2 Antibody, clone 14C7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: RSPO2, UNQ9384/PRO34209 UniProt Number: Q6UXX9
MABS1709-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1709-25UG
DescriptionAnti-R-Spondin 2 Antibody, clone 14C7.1
Alternate Names
  • Roof plate-specific spondin-2
  • hRspo2
Background InformationR-spondin-2 (UniProt: Q6UXX9; also known as Roof plate-specific spondin-2, hRspo2) is encoded by the RSPO2 (also known as UNQ9384/PRO34209) gene (Gene ID: 340419) in human. R-spondin-2 is a secreted protein that acts as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR receptors. Upon binding to LGR receptors (LGR4, LGR5 or LGR6) these receptors associate with phosphorylated LRP6 and frizzled receptors, which are activated by extracellular Wnt receptors. These events trigger the canonical Wnt signaling pathway to increase expression of target genes. R-spondin-2 also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling pathway by acting as an inhibitor of ZNRF3, an E3 ubiquitin-protein ligase that serves as an important regulator of the Wnt signaling pathway. In humans, R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lungs and is reported to downregulated in tumors involving these organs. In the embryonic mice it is abundantly expressed in the hippocampus and in developing bones.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-R-Spondin 2, clone 14C7.1, Cat. No. MABS1709, is a mouse monoclonal antibody that specifically targets R-Spondin 2 (RSPO2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution of this antibody detected RSPO2 in human pancreas, human kidney, and human bone marrow tissue.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 74 amino acids from the C-terminal end of human R-Spondin 2.
Clone14C7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14C7.1 specifically detects human R-spondin-2. It targets an epitope within 74 amino acids from the C-terminal end.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • RSPO2
  • UNQ9384/PRO34209
Purification MethodProtein G purified
UniProt Number
Molecular Weight~35 kDa observed; 28.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human recombinant R-spondin 2 protein.
Western Blotting Analysis: 0.02 µg/mL of this antibody detected 0.05 µg of human recombinant R-spondin 2 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1709-25UG 04054839522222