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MABE343-AF647 Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
MABE343-AF647
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue NumberMABE343-AF647
DescriptionAnti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody
Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenBacteria expressing puromycin-incorporated neosynthesized proteins at multiple molecular weights.
Clone12D10
Concentration0.5 mg/mL
HostMouse
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with cells from all species when pre-incubated with puromycin.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightRefer to Cat. No. MABE343 for observed molecular weight information.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE343-AF647 04053252976308

Documentation

Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody Certificates of Analysis

TitleLot Number
Anti-Puromycin, clone 12D10, Alexa -2814777 2814777
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 2991421
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 3012162
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 2952678
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 2922411
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate 3062299
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - Q2431444 Q2431444
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - 2906386 2906386
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - 2887487 2887487
Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate - 3121740 3121740
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies