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AB15302-I-25UG Anti-ProSAP2

Anti-ProSAP2, Cat. No. AB15302-I, is a highly specific rabbit polyclonal antibody that targets SH3 and multiple ankyrin repeat domains protein 3 and has been testedfor use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-ProSAP2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Shank3, Prosap2 UniProt Number: Q9JLU4
AB15302-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB15302-I-25UG
Brand Family Chemicon®
DescriptionAnti-ProSAP2
Alternate Names
  • SH3 and multiple ankyrin repeat domains protein 3
  • Shank3
  • Proline-rich synapse-associated protein 2
  • SPANK-2
Background InformationSH3 and multiple ankyrin repeat domains protein 3 (UniProt: Q9JLU4; also known as Shank3, Proline-rich synapse-associated protein 2, ProSAP2, SPANK-2) is encoded by the Shank3 (also known as Prosap2) gene (Gene ID: 59312) in rat. ProSAP2 belongs to SHANK family of proteins that is widely expressed in the brain. It serves as a major scaffold postsynaptic density (PSD) protein that interacts with multiple proteins and complexes to coordinate the dendritic spine and synapse formation, maturation and maintenance. It is reported to interconnect receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. ProSAP2 plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. It may be required at an early stage of synapse formation and be inhibited by IGF-1 to promote synapse maturation. ProSAP2 interacts with ankyrin repeats via its N-terminal region (aa 1-75). Its SH3-binding domain is localized to amino acids 1411-1417.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ProSAP2, Cat. No. AB15302-I, is a highly specific rabbit polyclonal antibody that targets SH3 and multiple ankyrin repeat domains protein 3 and has been testedfor use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected ProSAP2 in human cerebellum, mouse cerebral cortex, rat cerebral cortex, rat cerebellum, and rat spinal cord tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of rat Proline-rich synapse-associated protein 2 (ProSAP2). The immunogen sequence is conserved in all three isoforms.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Proline-rich synapse-associated protein 2 (ProSAP2) in rat, human, and murine cells. It targets an epitope within 16 amino acids from the N-terminal half.
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Shank3
  • Prosap2
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~193 kDa observed; 185.51 kDa and 186.38 kDa calculated for isoforms 1 and 2, respectively. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: 1 µg/mL of this antibody detected ProSAP2 in 10 µg of rat brain microsomal preparation.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB15302-I-25UG 04054839431708