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ABE863-25UL Anti-PHF1

Anti-PHF1, Cat. No. ABE863, is a highly specific rabbit polyclonal antibody that targets PHD finger protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PHF1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: PHF1, PCL1 UniProt Number: O43189
ABE863-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE863-25UL
DescriptionAnti-PHF1
Alternate Names
  • Information PHD finger protein 1
  • hPHF1
  • Polycomb-like protein 1
  • hPCl1
Background InformationInformation PHD finger protein 1 (UniProt: O43189; also known as PHF1, hPHF1, Polycomb-like protein 1, hPCl1) is encoded by the PHF1 (also known as PCL1) gene (Gene ID: 5252) in human. PHF1 is a member of the polycomblike family and contains two PHD-type zinc fingers (aa 87-142 and 186-240) and one Tudor domain (aa 29-86). It is essential for epigenetic regulation and genome maintenance. It specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) via its Tudor domain and recruits the PRC2 complex. PHF1 is highly expressed in heart, skeletal muscle, and pancreas, with lower levels found in brain, placenta, lung, liver and kidney. It is involved in DNA damage response and is recruited at double-strand breaks. PHF1 localizes to the promoters of numerous target genes and to double-strand breaks (DSBs) sites following DNA damage. It has been reported that PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. A chromosomal aberration involving PHF1 is reported to be the causative factor in endometrial stromal tumors. (Ref.: Sarma, K et al. (2008). Mol. Cell. Biol. 28(8): 2718-31; Musselman, CA et al (2012). Nat. Struct. Mol. Biol. 19(12):1266-72).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PHF1, Cat. No. ABE863, is a highly specific rabbit polyclonal antibody that targets PHD finger protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PHF1 in human pancreas and human kidney tissues.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected PHF1 in 10 µg of HeLa cell lysate.
Biological Information
ImmunogenGST-tagged recombinant fraction corresponding to 137 amino acids from the internal region of human PHD finger protein 1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Information PHD finger protein 1 (PHF1) in human cells. It targets an epitope within 137 amino acids from the internal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • PHF1
  • PCL1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell nuclear extract.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PHF1 in 10 µg of HeLa cell nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE863-25UL 04054839480171