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PC376 Anti-P2X4 Receptor (373-388) Rabbit pAb

Anti-P2X4 Receptor (373-388) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC376
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~65 kDa P2X4 receptor protein in mouse brain.
Catalogue NumberPC376
Brand Family Calbiochem®
Application Data
Detection of mouse P2X4 receptor by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml) pre-incubated without peptide (lane A) or with peptide control (25 µg/ml, lane B). Detection: chemiluminescence.

Detection of mouse P2X4 receptor by staining floating sections. Sample: Mouse cortex tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml). Detection: DAB
References
ReferencesLe, K.-T., et al. 1998. Neuroscience 83, 177.
Nicke, A., et al. 1998. EMBO J. 17, 3016.
Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
Buell, G., et al. 1996. EMBO J. 15, 55.
Collo, G., et al. 1996. J. Neurosci. 16, 2495.
Seguela, P., et al. 1996. J. Neurosci. 16, 448.
Bo, X., et al. 1995. FEBS Lett. 375, 129.
Lewis, C., et al. 1995. Nature 377, 432.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlMouse brain, rat brain, or SK-N-SH cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Application NotesFrozen Sections (1-2.5 µg/ml)
Immunoblotting (5 µg/ml)
Application CommentsA protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC376 0

Documentation

Anti-P2X4 Receptor (373-388) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-P2X4 Receptor (373-388) Rabbit pAb Certificates of Analysis

TitleLot Number
PC376

References

Reference overview
Le, K.-T., et al. 1998. Neuroscience 83, 177.
Nicke, A., et al. 1998. EMBO J. 17, 3016.
Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
Buell, G., et al. 1996. EMBO J. 15, 55.
Collo, G., et al. 1996. J. Neurosci. 16, 2495.
Seguela, P., et al. 1996. J. Neurosci. 16, 448.
Bo, X., et al. 1995. FEBS Lett. 375, 129.
Lewis, C., et al. 1995. Nature 377, 432.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-January-2009 JSW
ApplicationFrozen Sections (1-2.5 µg/ml)
Immunoblotting (5 µg/ml)
Application Data
Detection of mouse P2X4 receptor by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml) pre-incubated without peptide (lane A) or with peptide control (25 µg/ml, lane B). Detection: chemiluminescence.

Detection of mouse P2X4 receptor by staining floating sections. Sample: Mouse cortex tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-P2X4 Receptor (373-388) Rabbit pAb (Cat. No. PC376) (2 µg/ml). Detection: DAB
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~65 kDa P2X4 receptor protein.
BackgroundExtracellular ATP acts as a signaling molecule to mediate a wide variety of physiological responses through interactions with the P2 family of receptors. P2 receptors have been classified into two groups, the P2X family of ligand-gated ion channels, and the P2Y family of metabotropic G-protein coupled receptors. To date, seven P2X receptor subtypes and eight different P2Y receptor subtypes have been identified. The P2X receptor subunits exhibit approximately 35%-48% identity with each other at the amino acid level, and have been shown to form both homo- and hetereromultimeric complexes. The various P2X receptor subunits exhibit a broad pattern of distribution, being localized to different types of immune cells, glands, smooth muscle, skeletal muscle, and the central and peripheral nervous systems. The P2X4 receptor subunit is predominantly expressed in the central nervous system and has a widespread distribution as determined by in situ hybridization and immunohistochemistry.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH
IsotypeIgG
Specieshuman, mouse, rat
Positive controlMouse brain, rat brain, or SK-N-SH cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsA protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesLe, K.-T., et al. 1998. Neuroscience 83, 177.
Nicke, A., et al. 1998. EMBO J. 17, 3016.
Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev. 50, 413.
North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol. 7, 346.
Radford, K.M., et al. 1997. J. Neurosci. 17, 6529.
Buell, G., et al. 1996. EMBO J. 15, 55.
Collo, G., et al. 1996. J. Neurosci. 16, 2495.
Seguela, P., et al. 1996. J. Neurosci. 16, 448.
Bo, X., et al. 1995. FEBS Lett. 375, 129.
Lewis, C., et al. 1995. Nature 377, 432.

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