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ABS2248-100UL Anti-OVCH2

This rabbit polyclonal Anti-OVCH2, Cat. No. ABS2248, is a rabbit polyclonal antibody that detects Ovochymase-2 (OVCH2) and is tested for use in Immunohistochemistry and Western Blotting.

ABS2248-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2248-100UL
DescriptionAnti-OVCH2
Alternate Names
  • Ovochymase-2
  • Oviductin
Background InformationOvochymase-2 (UniProt: Q7M761; Oviductin, OVCH2) is encoded by the Ovch2 (also known as Ovtn) gene (Gene ID: 244199) in murine species. OVCH2 is a member of the peptidase S1 family that is synthesized with a signal peptide (aa 1-22) that is subsequently cleaved off. It also has a propeptide (aa 23-51) that serves as an activation peptide. OVCH2 is mainly localized in the caput region of epididymis that is most proximal to the testis. It is shown to be essential for processing ADAM3, the sperm surface protein that is required for the fertilizing ability of sperm. It is reported that a neural epidermal growth factor like like 2 (NELL2), binds to an orphan receptor tyrosine kinase, c-Ros oncogene 1 (ROS1), and mediates the differentiation of the initial segment (IS) of the caput epididymis. Male mice with NELL2 are reported to be infertile. (Ref.: Kiyozumi, D., et al. (2020). Science. 368(6495); 1132-1135).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody in 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis rabbit polyclonal Anti-OVCH2, Cat. No. ABS2248, is a rabbit polyclonal antibody that detects Ovochymase-2 (OVCH2) and is tested for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected OVCH2 in mature mouse caput epididymis lysate (Courtesy of Dr. Masahito Ikawa Lab @ Osaka University, Japan).

Western Blotting Analysis: A representative lot detected OVCH2 in Western Blotting applications (Kiyozumi, D., et. al. (2020). Science. 368(6495):1132-1135).

Immunohistochemistry Applications: A representative lot detected OVCH2 in Immunohistochemistry applications (Kiyozumi, D., et. al. (2020). Science. 368(6495):1132-1135).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 179 amino acids from the C-terminal region of murine Ovochymase-2 (OVCH2).
EpitopeC-terminal
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects murine Ovochymase-2 (OVCH2). It targets an epitope within the CUB2 domain from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ovch2
  • Ovtn
Purification MethodUnpurified
UniProt Number
Molecular Weight~74 kDa observed; 67.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mature mouse caput epididymis lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OVCH2 in mature mouse caput epididymis lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2248-100UL 04065266298260