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MAB1277-I-100UG Anti-Nucleoli Antibody, clone 125-10

Anti-Nucleoli, clone 125-10, Cat. No. MAB1277-I, is a mouse monoclonal antibody that detects nucleoli and is tested for use in Immunofluorescence and Immunocytochemistry.

MAB1277-I-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB1277-I-100UG
DescriptionAnti-Nucleoli Antibody, clone 125-10
Alternate Names
  • Nucleolus
Background InformationThe nucleoli small basophilic, spherical, non-membrane enclosed, highly conserved, structures that are localized within the nucleus. Usually, they are localized in the central nuclear region or close to the nuclear membrane. They are formed around nucleolus organizer regions (NORs) consisting of ribosomal DNA (rDNA). They are structurally organized into the fibrillar center, the dense fibrillar component, and the granular component region. Clone 125-10 detects human nucleoli by immunocytochemistry.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-Nucleoli, clone 125-10, Cat. No. MAB1277-I, is a mouse monoclonal antibody that detects nucleoli and is tested for use in Immunofluorescence and Immunocytochemistry.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected Nucleoli in Immunocytochemistry applications (Endo, A., et al. (2009). J Biol Chem. 284(41):27918-27923; Endo, A., et al. (2009). J Cell Sci. 122(Pt 5):678-86).

Immunofluorescence Analysis: A representative lot detected Nucleoli in Immunofluorescence applications (Clements, D., et al. (2007). Am J Physiol Lung Cell Mol Physiol. 292(1):L258-66; Vallee, D., et al. (2004). J Med Genet. 41(10):778-83; Ohno, M., et al. (2002). Chromosoma. 111(3):201-13).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRaji cell nuclei.
EpitopeUnknown
Clone125-10
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 125-10 is a mouse monoclonal antibody that specifically detects nucleoli in human cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Nucleoli in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1277-I-100UG 04065267404066