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AB5664P Anti-Neurite Outgrowth Inhibitor A Antibody

Anti-Neurite Outgrowth Inhibitor A Antibody is an antibody against Neurite Outgrowth Inhibitor A for use in ELISA & WB.

Anti-Neurite Outgrowth Inhibitor A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins, Growth Cones & Axon Guidance Gene Symbol: RTN4, RTN4-B1, Nbla10545, RTN-X, reticulon-4A, Nogo-C, NSP, RTN-x, ASY, foocen, NSP-CL, NOGOC, RTN4-A, Reticulon-4, Nbla00271, KIAA0886, NOGOA, Reticulon-5, Foocen, NI220/250, RTN4-C, RTN4-B2, SP1507, NOGO-A, NOGO, NOGOB, Nogo-B UniProt Number: Q9NQC3
AB5664P
50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5664P
ReplacesAB5888
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Neurite Outgrowth Inhibitor A Antibody
Alternate Names
  • Nogo-A
References
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.
Quality LevelMQ100
Applications
ApplicationAnti-Neurite Outgrowth Inhibitor A Antibody is an antibody against Neurite Outgrowth Inhibitor A for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1-10 μg/mL using ECL. The calculated molecular weight of Nogo-A is approximately 135 kDa. However a molecular weight of approximately 180 kDa has been reported for full length Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog Number AG348) per well.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenAn 18 amino acid peptide sequence in the middle region of the putative extracellular domain of rat Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

The immunogen peptide is highly conserved in mouse (94%) and human (83%) Nogo-A.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes rat Neurite Outgrowth Inhibitor Protein A (Nogo-A). The immunogen shows no significant sequence homology with the alternatively spliced shorter forms Nogo-B or Nogo-C.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
Gene Symbol
  • RTN4
  • RTN4-B1
  • Nbla10545
  • RTN-X
  • reticulon-4A
  • Nogo-C
  • NSP
  • RTN-x
  • ASY
  • foocen
  • NSP-CL
  • NOGOC
  • RTN4-A
  • Reticulon-4
  • Nbla00271
  • KIAA0886
  • NOGOA
  • Reticulon-5
  • Foocen
  • NI220/250
  • RTN4-C
  • RTN4-B2
  • SP1507
  • NOGO-A
  • NOGO
  • NOGOB
  • Nogo-B
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9NQC3 # Potent neurite growth inhibitor in vitro and plays a role both in the restriction of axonal regeneration after injury and in structural plasticity in the CNS. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
SIZE: 1192 amino acids; 129931 Da
SUBUNIT: Binds to RTN4R. Interacts with Bcl-xl and Bcl-2. Isoform 2 binds to NGBR and RTN3. Isoform 2 and isoform 3 interact with BACE1 and BACE2.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
TISSUE SPECIFICITY: Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific.
DOMAIN: SwissProt: Q9NQC3 Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo- 66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor (By similarity).
SIMILARITY: Contains 1 reticulon domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months afet date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5664P 04053252271625

Documentation

Anti-Neurite Outgrowth Inhibitor A Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Neurite Outgrowth Inhibitor A Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3176790 3176790
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2459028 2459028
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3284359 3284359
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3420937 3420937
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3609368 3609368
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3888768 3888768
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3943880 3943880
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) -2719862 2719862
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) -2769573 2769573
RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) -2776255 2776255
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