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475985 Anti-Myosin, Smooth Muscle Heavy Chain Mouse mAb (SMMS-1)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
475985
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
OverviewRecognizes the ~205 kDa myosin heavy chain-1 and the ~200 kDa myosin heavy chain-2 in myoepithelial cell layers in benign and in situ malignant breast lesions.
Catalogue Number475985
Brand Family Calbiochem®
References
ReferencesBabu, G.J., et al. 2000. Microsc. Res. Tech. 15, 532. Watchko, J.F., et al. 1998. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 119, 459. Small, J.V. 1995. Bioessays 17, 785. Price, R.J., et al. 1994. Circ. Res. 75, 520. Frid, M.G., et al. 1992. Dev. Biol. 153, 185. Glukhova, M.A., et al. 1990. J. Biol. Chem. 265, 13042.
Product Information
FormLiquid
FormulationIn PBS, 0.2% BSA, pH 7.4.
Positive controlMyoepithelial cell layers in benign and malignant breast tumors
Preservative≤0.1% sodium azide
Applications
Key Applications Paraffin Sections
Application NotesParaffin Sections (1-2 µg/ml, trypsin and heat pre-treatment required)
Application CommentsStaining of formalin-fixed tissues requires digesting sections with trypsin at 1 mg/ml PBS for 10 min at 37°C, followed by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena crude extract from human uterus
ImmunogenHuman
CloneSMMS-1
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
475985 0

Documentation

Anti-Myosin, Smooth Muscle Heavy Chain Mouse mAb (SMMS-1) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Babu, G.J., et al. 2000. Microsc. Res. Tech. 15, 532. Watchko, J.F., et al. 1998. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 119, 459. Small, J.V. 1995. Bioessays 17, 785. Price, R.J., et al. 1994. Circ. Res. 75, 520. Frid, M.G., et al. 1992. Dev. Biol. 153, 185. Glukhova, M.A., et al. 1990. J. Biol. Chem. 265, 13042.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
ApplicationParaffin Sections (1-2 µg/ml, trypsin and heat pre-treatment required)
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~205 kDa myosin heavy chain-1 and the ~200 kDa myosin heavy chain-2.
BackgroundSmooth muscle is an important element of the vascular, digestive, urogenital and respiratory systems. Smooth muscle cells (SMCs) are spindle-shaped cells that contain a variety of obliquely arrayed actomyosin-rich contractile elements that are attached to the plasma membrane. Smooth muscle heavy chain (SM-MHC) is a structural protein that is found in the cytoplasm. SM-MHC is a major component of the contractile apparatus in smooth muscle cells. Alternative splicing of the primary transcript from a single gene generates four smooth muscle myosin heavy chain isoforms. These isoforms differ both at the carboxyl terminus (SM1 and SM2 isoforms) and at the amino terminus (SM-A and SM-B isoforms). The smooth muscle myosin heavy chain isoforms are differentially expressed during smooth muscle development and in different smooth muscle cell types.
HostMouse
Immunogen speciesHuman
Immunogena crude extract from human uterus
CloneSMMS-1
IsotypeIgG₁
Specieshuman
Positive controlMyoepithelial cell layers in benign and malignant breast tumors
FormLiquid
FormulationIn PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsStaining of formalin-fixed tissues requires digesting sections with trypsin at 1 mg/ml PBS for 10 min at 37°C, followed by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesBabu, G.J., et al. 2000. Microsc. Res. Tech. 15, 532. Watchko, J.F., et al. 1998. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 119, 459. Small, J.V. 1995. Bioessays 17, 785. Price, R.J., et al. 1994. Circ. Res. 75, 520. Frid, M.G., et al. 1992. Dev. Biol. 153, 185. Glukhova, M.A., et al. 1990. J. Biol. Chem. 265, 13042.