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MABF2755-25UL Anti-Myeloid lineage Antibody, clone OX-82

Anti-Myeloid lineage, clone OX-82, Cat. No. MABF2755, is a mouse monoclonal antibody that detests cells of the Myeloid lineage and is tested for use in Flow Cytometry and Fluorescence Activated Cell Sorting (FACS),

MABF2755-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF2755-25UL
DescriptionAnti-Myeloid lineage Antibody, clone OX-82
Background InformationHematopoietic stem cells (HSC) are known to differentiate either into lymphoid cells or and myeloid cells that include granulocytes, monocytes, megakaryocytes, and dendritic cells. During hematopoiesis, lymphoid lineage cells originate from the lymph tissue and myeloid lineage cells originate from the bone marrow. Hematopoiesis is shown to change over time, with a bias of the dominating HSC towards myeloid development with advancing age. Defects in myelopoiesis is reported to result in several hematologic disorders. Clone OX-82 identifies myeloid cells and stromal elements in a variety of tissues in the adult. It reacts with a 35 kDa molecule expressed on myeloid cells and is useful in isolating pluripotent stem cells from rat bone marrow. (Ref.: Weiskopf, K., et al. (2016). Microbiol Spectr. 4(5); doi:10.1128; Crook, K., and Hunt, SV. (1996). Dev. Immunol. 4(4); 235-246).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Myeloid lineage, clone OX-82, Cat. No. MABF2755, is a mouse monoclonal antibody that detests cells of the Myeloid lineage and is tested for use in Flow Cytometry and Fluorescence Activated Cell Sorting (FACS),
Key Applications
  • Flow Cytometry
  • Fluorescence Activated Cell Sorting (FACS)
Application NotesTested applications

Fluorescence Activated Cell Sorting (FACS): A representative lot detected Myeloid lineage in Fluorescence Activated Cell Sorting applications (Crook, K., et al. (1996). Dev Immunol. 4(4):235-46).

Flow Cytometry Analysis: A representative lot detected Myeloid lineage in Flow Cytometry applications (Crook, K., et al. (1996). Dev Immunol. 4(4):235-46; Shaaban, S., et al. (2016). Int J Cancer Res. 12(2):69-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenAnemic rat bone marrow cells.
EpitopeExtracellular domain
CloneOX-82
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone OX-82 is a mouse monoclonal antibody that detects Myeloid lineage cells.
IsotypeIgG1
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Rat neutrophils.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Myeloid lineage in one million Rat neutrophils.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2755-25UL 04065270172495