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AB3540P Anti-Monocarboxylate Transporter 1 Antibody

AB3540P
50 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RELISA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3540P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Monocarboxylate Transporter 1 Antibody
Alternate Names
  • MCT1
Product Information
FormatAffinity Purified
PresentationLiquid in PBS containing 0.1% BSA .
Quality LevelMQ100
Applications
ApplicationDetect Monocarboxylate Transporter 1 using this Anti-Monocarboxylate Transporter 1 Antibody validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1-5 μg/mL using ECL. Membrane preps are recommended.



ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenA 17 amino acid peptide sequence near the C-terminus of rat MCT1 (Gerhart et al. 1997; Knott et al. 1999; Takanaga et al. 1995; Carpenter et al. 1996; Koehler-Stec et al. 1998; Yoon et al. 1999; Halestrap & Price 1999).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes rat Monocarboxylate Transporter 1 (MCT1). The immunogen shows no significant sequence homology with MCT2-8.

SPECIES REACTIVITIES: The immunogen sequence is 93% conserved in hamster, 80% in mouse and only 40% in human. Reactivity with other species has not been confirmed.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM]
Gene Symbol
  • SLC16A1
  • MCT
  • FLJ36745
  • MGC44475
  • MCT1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P53985 # Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
SIZE: 500 amino acids; 53958 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in normal and in cancer cells.
DISEASE: SwissProt: P53985 # Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT) [MIM:245340]; also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals.
SIMILARITY: SwissProt: P53985 ## Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
AB3540P 04053252319815