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MABE1023 Anti-Mitotic Checkpoint Protein BUB3 Antibody, clone 1A3.1

This Anti-Mitotic Checkpoint Protein BUB3 Antibody, clone 1A3.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Mitotic Checkpoint Protein BUB3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: BUB3, BUB3L UniProt Number: O43684
MABE1023
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABE1023
DescriptionAnti-Mitotic Checkpoint Protein BUB3 Antibody, clone 1A3.1
Alternate Names
  • Mitotic checkpoint protein BUB3
  • Budding uninhibited by benomyl
  • Budding uninhibited by benzimidazoles 3 homolog
  • hBUB3
Background InformationMitotic checkpoint protein BUB3 (UniProt O43684; also known as Budding uninhibited by benomyl, Budding uninhibited by benzimidazoles 3 homolog, hBUB3) is encoded by the BUB3 (also known as BUB3L) gene (Gene ID 9184) in human. The mitotic checkpoint is activated when there is unattached kinetochores to spindle microtubules. When all kinetochores are attached to spindle microtubules and bi-orientation of sister chromatids is established, the mitotic checkpoint signaling is silenced and chromosome segregation proceeds. BUB3 is a key mitotic checkpoint factor that recognizes centromeric components and recruits other mitotic checkpoint molecules to the unattached kinetochore, where they assemble as an active complex. BUB3 interacts with BUB1, BubR1, MAD2, and CDC20 through its WD repeat, while the region 213-EVQKKKYAFK-222 is reported to be essential for proper BUB3 nuclear localization and its interaction with CENP-A and KNL1.
References
Product Information
FormatCulture Supernatant
PresentationMouse monoclonal IgG2bκ antibody in supernatant without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Mitotic Checkpoint Protein BUB3 Antibody, clone 1A3.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Mitotic Checkpoint Protein BUB3.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A 1:250 dilution from a representative lot detected Mitotic Checkpoint Protein BUB3 in 10 µg of HeLa lysate.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected Mitotic Checkpoint Protein BUB3 in HUVECs and HeLa cells.
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to the WD repeats 6 & 7 of human BUB3.
EpitopeWD repeats 6 & 7.
Clone1A3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms reported by UniProt (O43684).
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BUB3
  • BUB3L
Purification MethodUnpurified
UniProt Number
Molecular Weight~37 kDa observed. 37.16 kDa (isoform 1) and 36.96 kDa (isoform 2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C2C12 mouse myoblast lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected Mitotic Checkpoint Protein BUB3 in 10 µg of C2C12 mouse myoblast lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1023 04055977314939