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ABE2585-25UG Anti-Mis12 Complex

Anti-Mis12 Complex Antibody, Cat. No. ABE2585-25UG, is a highly specific rabbit polyclonal antibody, that targets Mis12 complex and has been tested in Western Blotting and Immunofluorescence and Immunocytochemistry.

Anti-Mis12 Complex MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MIS12, PMF1, DSN1, NSL1 UniProt Number: Q9H081, Q6P1K2, Q9H410, Q96IY1
ABE2585-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IF, ICCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2585-25UG
DescriptionAnti-Mis12 Complex
Background InformationMis12 complex is composed of four subunits, Protein MIS12 homolog, Polyamine-modulated factor 1, Kinetochore-associated protein DSN1 homolog, and Kinetochore-associated protein NSL1 homolog (UniProt: Q9H081, Q6P1K2, Q9H410, Q96IY1, respectively) that are encoded by genes known as MIS12 (Gene ID: 79003), PMF1 (Gene ID: 100527963), DSN1 (Gene ID: 79980), and NSL1 (also known as C1orf48, DC31, DC8, MIS14) (Gene ID: 25936) in human. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. This complex acts as the primary microtubule-binding interface at kinetochores and provides a platform to recruit regulatory proteins. In human Mis12 complex subunits are shown to localize with centromere protein A (CENP-A) at inner kinetochores and internally to Ndc80 at outer kinetochores. Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. Reduced levels of Mis12 complex proteins are shown to result in chromosome alignment defects in both human and chicken cells, but spindle bipolarity is not disturbed. (Ref.: Kline SL et al (2006). J. Cell. Biol. 173(1): 9-17).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS without azide and with 50% glycerol.
Applications
ApplicationAnti-Mis12 Complex Antibody, Cat. No. ABE2585-25UG, is a highly specific rabbit polyclonal antibody, that targets Mis12 complex and has been tested in Western Blotting and Immunofluorescence and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1,100 dilution from a representative lot detected Mis12 complex in HeLa cells.

Immunofluorescence Analysis: A representative lot detected Mis12 complex in HeLa cells (Rago, F., et. al. (2015). Curr Biol. 25(5):671-7).
Biological Information
ImmunogenFour subunits from human Mis12 complex.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Mis12 complex in human. It will also detect individual subunits (Mis12, PMF1, Dsn1, Nsl1) in Western Blotting.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • MIS12
  • PMF1
  • DSN1
  • NSL1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight40 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in recombinant Mis12 complex.
Western Blotting Analysis: 2.2 µg/mL   of this antibody detected Mis12 complex in 1 UL of recombinant  Mis12 complex.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2585-25UG 04054839464683