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MABC1607-25UG Anti-MMP-11 (Stromelysin-3) Antibody, clone 5ST-4A9

Detect Stromelysin-3 using this mouse monoclonal Anti-MMP-11 (Stromelysin-3), clone 5ST-4A9 Antibody, Cat. No. MABC1607-25UG, validated for use in Western Blotting Immunoprecipitation and Immunohistochemistry (Paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MMP11, STYM3 UniProt Number: P24347
MABC1607-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1607-25UG
DescriptionAnti-MMP-11 (Stromelysin-3) Antibody, clone 5ST-4A9
Alternate Names
  • Stromelysin-3
  • SL-3
  • ST3
  • MMP11
  • Matrixmetalloproteinase 11
Background InformationStromelysin-3 (UniProt: P24347; also known as SL-3, ST3, Matrix metalloproteinase-11, MMP-11) is encoded by the MMP11 (also known as STMY3) gene (Gene ID: 4320) in human. MMP-11 is shown to be differentially expressed in tumor tissue and plays a role in tumor invasion. It is shown to be well expressed in stromal cells of breast carcinomas. It has little expression in adult organs under normal physiological conditions. During development, it is expressed in many processes involved in cell migration and tissue morphogenesis. MMP-11 has multiple zinc and calcium binding sites. It binds 2 zinc ions per subunit and one calcium ion per subunit. MMP-11 contains 4 hemopexin domains (aa 291-339; 340-382; 384-432; and 433-480). It is synthesized with a signal peptide (aa 1-31) and a propeptide region (aa 32-97) that are cleaved to generate mature enzyme. It contains a conserved cysteine that is present in the cysteine-switch motif (aa 78-85) that binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion activates the enzyme. The precursor is cleaved by a furin endopeptidase. Expression levels of MMP-11 are a reliable indicator of greatest risk for cancer recurrence in breast carcinoma, pancreatic tumors, and colon cancer. (Ref.: Roscilli, G et al. (2014). J. Transl. Med. 12; 54).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Stromelysin-3 using this mouse monoclonal Anti-MMP-11 (Stromelysin-3), clone 5ST-4A9 Antibody, Cat. No. MABC1607-25UG, validated for use in Western Blotting Immunoprecipitation and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunohistochemistry applications in (Ahmad, A., et. al. (1998) Am J Pathol. 152(3):721-8; Nakopoulou, L., et. al. (2002). Mod Pathol . 15(11):1154-61).
Western Blotting Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Western Blotting applications in (von Marschall, Z., et. al. (1998) Gut. 43(5):692-8; Motrescu, E.R., et. al. (2008). Oncogene. 27(49):6347-55; Luo, D., et. al. (1999). J Biol Chem. 274(52):37177-85).
Immunohistochemistry Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunohistochemistry applications in (Cribier, B., et. al. (2002). J Am Acad Dermatol . 46(3):408-13; von Marschall, Z., et. al. (1998) Gut. 43(5):692-8; Andarawewa, K.L., et. al. (2005). Cancer Res. 65(23):10862-71; Motrescu, E.R., et. al. (2008). Oncogene. 27(49):6347-55.
Immunoprecipitation Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunoprecipitation applications in (Schonbeck, U., et. al. (1999). J Exp Med. 189(5):843-53).
Immunohistochemistry Analysis: A 1:50, 250, 1,000 dilution from a representative detected MMP-11 (Stromelysin-3) in human placenta and human breast cancer tissue.
Biological Information
ImmunogenHis-tagged full length recombinant human MMP-11 (Stromelysin-3).
Clone5ST-4A9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5ST-4A9 detects MMP-11 (stromelysin 3) in human cells.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MMP11
  • STYM3
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC-3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected MMP-11 (Stromelysin-3) in 10 µg of PC-3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1607-25UG 04054839437847