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MABE2032-25UG Anti-MLK1/MRTF-A Antibody, clone 1A11

Anti-MLK1/MRTF-A, clone 1A11, Cat. No. MABE2032, is a mouse monoclonal antibody that detects MLK1/MRTF-A and is tested for use in Immunofluorescence and Western Blotting.

MABE2032-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE2032-25UG
DescriptionAnti-MLK1/MRTF-A Antibody, clone 1A11
Alternate Names
  • Myocardin-related transcription factor A
  • MRTF-A
  • MKL/myocardin-like protein 1
  • Megakaryoblastic leukemia 1 protein
  • Megakaryocytic acute leukemia protein
Background InformationMyocardin-related transcription factor A (UniProt: Q969V6; also known as MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein) is encoded by the MRTFA (also known as KIAA1438, MAL, MLK1) gene (Gene ID: 57591) in human. MRTF-A is a ubiquitously expressed transcription coactivator that associates with serum response factor (SRF) transcription factor to control expression of genes that regulate cytoskeleton during development, morphogenesis, and cell migration. The MRTFA-SRF complex activity responds to Rho GTPase-induced changes in cellular G-actin concentration, and thereby couples cytoskeletal gene expression to cytoskeletal dynamics. It is reported to bind to G-actin via its RPEL repeats. It is detected in cytoplasm as well as in the nucleus and its subcellular location is tightly regulated by actin. High levels of G-actin in the nucleus, observed during serum deprivation, result in low levels of nuclear MRTF-A, while reduced levels of nuclear G-actin result in accumulation of MRTF-A in the nucleus. G-actin-binding in the cytoplasm inhibits its nuclear import by masking the nuclear localization signal (NLS). In contrast, binding to nuclear G-actin promotes its nuclear export to the cytoplasm. Phosphorylation of serine 6 by ERK inhibits the binding of G-actin, unmasking the NLS and promoting nuclear import. Its N-terminal region is required for its nuclear localization and the C-terminal region mediates its transcriptional activity. (Ref.: Weissbach, J., et al. (2016). Mol. Cell. Biol. 36(10); 1526-1539; Busche, S., et al. (2010). J. Cell Sci. 123(16); 2803-2809).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-MLK1/MRTF-A, clone 1A11, Cat. No. MABE2032, is a mouse monoclonal antibody that detects MLK1/MRTF-A and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected MLK1/MRTF-A in Immunofluorescence application (Busche, S., et al. (2010). J Cell Sci. 123(Pt16):2803-9).

Western Blotting Analysis: A representative lot detected MLK1/MRTF-A in Western Blotting application (Weissbach, J., et al. (2016). Mol Cell Biol. 36(10):1526-39).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 260 amino acids from the N-terminal region of human Myocardin-related transcription factor A (MLK1/MRTF-A).
EpitopeN-terminal
Clone1A11
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1A11 is a mouse monoclonal antibody that detects Myocardin-related transcription factor A (MLK1/MRTF-A). It targets an epitope within the N-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MRTFA
Purification MethodProtein G purified
UniProt Number
Molecular Weight~140 kDa observed; 98.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MLK1/MRTF-A in Ramos cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE2032-25UG 04065270451750