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ABE1338 Anti-METTL14 Antibody

This Anti-METTL14 Antibody is validated for use in Western Blotting for the detection of METTL14.

Anti-METTL14 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: METTL14, KIAA1627 UniProt Number: Q9HCE5
ABE1338
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1338
DescriptionAnti-METTL14 Antibody
Alternate Names
  • N6-adenosine-methyltransferase subunit METTL14
  • m6A methyltransferase METTL14
  • Methyltransferase-like protein 14
Background InformationN6-adenosine-methyltransferase subunit METTL14 (EC 2.1.1.62; UniProt Q9HCE5; also known as m6A methyltransferase METTL14, Methyltransferase-like protein 14) is encoded by the METTL14 (also known as KIAA1627) gene (Gene ID 57721) in human. N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification in messenger and long noncoding RNAs. This reversible modification is installed by the m(6)A methylation "writers" and remomved by demethylases that serve as "erasers. In addition, methyl-specific binding proteins, primarily of the YTH-domain family, function as "readers" and mediate the effect by binding to modified transcripts. METTL14 is a component of the nuclear methyltransferase complex (writers) composed of METTL3, METTL14, and WTAP. At least 2 demethylases (erasers), ALKBH5 and FTO, that can catalyze the reverse reaction. The roles of m(6)A in cell fates regulation and embryonic development highlight the existence of another layer of epigenetic regulation at the RNA level.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-METTL14 Antibody is validated for use in Western Blotting for the detection of METTL14.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence from the C-terminal half of human METTL14.
EpitopeInternal (C-terminal half).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThe immunogen sequence is present in human METTL14 (UniProt Q9HCE5) and both of murine METTL14 spliced isoforms (Uniprot Q3UIK4).
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Zebrafish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • METTL14
  • KIAA1627
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~56 kDa observed. 52.15 kDa (human), 52.12/40.72 kDa (mouse isoform 1/2) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected METTL14 in 10 µg of Raji nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1338 04055977355376