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ST1116 Anti-MAVS Rabbit pAb

Anti-MAVS Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cardif pAb, Anti-VISA pAb, Anti-IPS-1 pAb

ST1116
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~56 kDa MAVS protein in Saos-2 cells.
Catalogue NumberST1116
Brand Family Calbiochem®
SynonymsAnti-Cardif pAb, Anti-VISA pAb, Anti-IPS-1 pAb
Application Data
Lane 1: Detection of human MAVS by immunoblotting. Sample: Whole cell lysate (20 µg) from Saos-2 cells. Primary antibody: Anti-MAVS Rabbit pAb (Cat. No. ST1116) (0.1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of MAVS by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from Saos2 cells. Antibody for immunoprecipitation: Anti MAVS Rabbit pAb (Cat No. ST1116) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated MAVS. Primary antibody: Anti-MAVS Rabbit pAb (Cat No. ST1116) (0.1 µg/ml). Detection: chemiluminescence.
References
ReferencesSeth, R.B., et al. 2005. Cell 122, 669.
McWhirter, S.M., et al. 2005. Cell 122, 645.
Meylan, E., et al. 2005. Nature 437, 1167.
Kawai, T., et al. 2005. Nat. Immunol. 6, 981.
Sun, Q., et al. 2006. Immunity 24, 633.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
Positive controlSaos-2 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (0.1 µg/ml)
Immunoprecipitation (10 µg/mg total protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids in middle region of human MAVS
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1116 0

Documentation

References

Reference overview
Seth, R.B., et al. 2005. Cell 122, 669.
McWhirter, S.M., et al. 2005. Cell 122, 645.
Meylan, E., et al. 2005. Nature 437, 1167.
Kawai, T., et al. 2005. Nat. Immunol. 6, 981.
Sun, Q., et al. 2006. Immunity 24, 633.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-October-2007 RFH
SynonymsAnti-Cardif pAb, Anti-VISA pAb, Anti-IPS-1 pAb
ApplicationImmunoblotting (0.1 µg/ml)
Immunoprecipitation (10 µg/mg total protein)
Application Data
Lane 1: Detection of human MAVS by immunoblotting. Sample: Whole cell lysate (20 µg) from Saos-2 cells. Primary antibody: Anti-MAVS Rabbit pAb (Cat. No. ST1116) (0.1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of MAVS by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from Saos2 cells. Antibody for immunoprecipitation: Anti MAVS Rabbit pAb (Cat No. ST1116) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated MAVS. Primary antibody: Anti-MAVS Rabbit pAb (Cat No. ST1116) (0.1 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinty purified rabbit polyclonal antibody. Recognizes the ~56 kDa MAVS protein.
BackgroundMAVS (mitochondrial antiviral signaling, accession no. NP_065797) is essential for NF-κB and IRF3 activation by RNA viruses. MAVS contains an N-terminal CARD-like domain and a C-terminal transmembrane domain that targets the protein to the mitochondrial membrane. The mitochondrial targeting transmembrane domain is essential for MAVS signaling, implicating a role for mitochondria in innate immunity. Suppression of MAVS expression blocks interferon production and exacerbates viral replication and killing of host cells. Overexpression of MAVS augments interferon production and confers antiviral immunity. MAVS-deficient cells are compromised in viral-dependent induction of interferons and activation of NF-κB. MAVS-deficient mice fail to induce interferons in response to poly(I:C) stimulation.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids in middle region of human MAVS
IsotypeIgG
Specieshuman
Positive controlSaos-2 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSeth, R.B., et al. 2005. Cell 122, 669.
McWhirter, S.M., et al. 2005. Cell 122, 645.
Meylan, E., et al. 2005. Nature 437, 1167.
Kawai, T., et al. 2005. Nat. Immunol. 6, 981.
Sun, Q., et al. 2006. Immunity 24, 633.