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05-337 Anti-MARCKS Antibody, clone 2F12

Anti-MARCKS Antibody, clone 2F12 detects level of MARCKS & has been published & validated for use in IP & WB.

Anti-MARCKS Antibody, clone 2F12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: MARCKS, 80K-L, MACS, PRKCSL, FLJ14368, phosphomyristin, MRACKS, PKCSL, FLJ90045 UniProt Number: P29966
05-337
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HIP, WBMAscitesMonoclonal Antibody
Description
Catalogue Number05-337
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-MARCKS Antibody, clone 2F12
References
Product Information
FormatAscites
Presentationmouse ascites containing 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-MARCKS Antibody, clone 2F12 detects level of MARCKS & has been published & validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenPurified recombinant human MARCKS; Epitope mapped to the carboxyl-terminal.
Clone2F12
HostMouse
SpecificityMARCKS
IsotypeIgG1
Species Reactivity
  • Bovine
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Gene Symbol
  • MARCKS
  • 80K-L
  • MACS
  • PRKCSL
  • FLJ14368
  • phosphomyristin
  • MRACKS
  • PKCSL
  • FLJ90045
Purification MethodAscites
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SIZE: 332 amino acids; 31555 Da
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
Molecular Weightnone given
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on bovine brain extract
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-337 08436037125508

Documentation

Anti-MARCKS Antibody, clone 2F12 SDS

Title

Safety Data Sheet (SDS) 

Anti-MARCKS Antibody, clone 2F12 Certificates of Analysis

TitleLot Number
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2135164 2135164
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2366548 2366548
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2382138 2382138
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2390491 2390491
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2392258 2392258
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2428644 2428644
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) 3050161
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2066049 2066049
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2196187 2196187
Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2198983 2198983
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References

Reference overviewApplicationPub Med ID
20-Hydroxyeicosatetraenoic acid-induced vasoconstriction and inhibition of potassium current in cerebral vascular smooth muscle is dependent on activation of protein kinase C
Lange, A., et al
J Biol Chem, 272:27345-52 (1997)  1997

Immunoblotting (Western)9341185 9341185

FAQ

QuestionAnswer
What is the concentration of this antibody?We are not able to quantitate ascites.
What is the molecular weight of MARCKS?MARCKS is a protein that can rang in apparent molecular weight from 32-92kDa depending on the cell or tissue extract. It's unique modified amino acid primary structure and multiple PKC phosphorylation sites account for the anomalous mobility during SDS-PAGE, which can explain the slight variance in apparent molecular weight.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies